Rabbit Polyclonal IgG Anti-Fibronectin, mouse
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Catalog numberFI24951-0.1
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PricePlease ask
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Size0.1 ml
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AvailabilityPlease contact us to check the availability
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ImmunogenPurified fibronectin from murine plasma
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SpecificityMurine fibronectin 100%, murine collagen type I, III and IV
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Species reactivityMouse
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Product presentationAffinity purified antibody lyophilized from phosphate buffered solution; no BSA and preservative added!
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Product categoryPrimary Antibody
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Antibody s raised inRabbit
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Antibody s isotypeIgG
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Antibody s clonepolyclonal
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Product is supplied aspurified, lyophilized
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Recommended concentration for useIFA ≥ 1:80, IHC(P) ≥ 1:1000, ELISA ≥ 1:200 (OD ≥ 500)
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Protein concentrationapp. 1 mg/ml
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Positive controlMouse skin
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PretreatmentAfter de-waxing the tissue slices they are treated with 0.2% hyaluronidase (app. 300 U/mg e.g. Art. No. HYA02-50) in TBS 15 min at 37°C. There after non-specific binding is blocked by blocking serum or 3% BSA in TBS. For peroxidase systems blocking with 1% peroxide solution in TBS for 30 min at RT is recommended.
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Tested applicationsIFA, IHC(P), ELISA, RIA
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Recommended secondary reagentAnti-rabbit IgG-conjugates, e.g. anti-rabbit IgG:FITC (Art. No. FI-1000) or anti-rabbit IgG:DyLight488 (Art. No. DI-1488). For paraffin sections best results have been obtained by use of DAKO Envision or VECTOR ImmPress systems (Art. No. MP-7401).
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Storage methodStore at -20°C
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UniProt numberP11276 (FINC_MOUSE)
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Litterature1. Andujar MB, Magloire H, Hartmann DJ, Ville G, Grimaud JA (1985) Early mouse molar root development: cellular changes and distribution of fibronectin, laminin and type-IV collagen. Differentiation. 30(2):111-22. 2.Savino W, Itoh T, Imhof BA, Dardenne M (1986) Immunohistochemical studies on the phenotype of murine and human thymic stromal cell lines. Thymus. 8(4):245-56. 3. Villa-Verde DM1, Lagrota-Candido JM, Vannier-Santos MA, Chammas R, Brentani RR, Savino W (1994) Extracellular matrix components of the mouse thymus microenvironment. IV. Modulation of thymic nurse cells by extracellular matrix ligands and receptors. Eur J Immunol. 24(3):659-64. 4. Pankov R and Yamada KM (2002) Fibronectin at a glance. J Cell Sci. Oct 15;115(Pt 20):3861-3 5. Labat-Robert J (2002) Fibronectin in malignancy. Semin Cancer Biol. Jun;12(3):187-95.
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DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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AboutImmunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures. Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera. Rabbits are used for polyclonal antibody production by bio logo. Rabbit antibodies are very stable and can be stored for several days at room temperature. bio logo adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
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TestMouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin nameMus musculus, Oryctolagus cuniculus
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GroupPolyclonals and antibodies
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Gene target
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Gene symbolFANK1, FNDC3A, FLRT3
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Short nameECM
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TechniquePolyclonal, Rabbit, anti-, Mouse, anti, IgG, antibody to, antibodies, IgGs, mouses, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
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HostRabbit, Rabbits
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IsotypeIgG, IgG
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SpeciesMouse, Mouses
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Alternative nameFibronectin, mouse
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Alternative techniquepolyclonals, rabbit-anti, murine, antibodies, immunoglobulins
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Gene info
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Identity
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Gene
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Long gene namefibronectin type III and ankyrin repeat domains 1
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Synonyms gene name
- fibronectin type 3 and ankyrin repeat domains 1
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GenBank acession
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Locus
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Discovery year2003-11-21
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Ankyrin repeat domain containing
- Fibronectin type III domain containing
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namefibronectin type III domain containing 3A
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Synonyms gene
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Synonyms gene name
- fibronectin type III domain containing 3
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Synonyms
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GenBank acession
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Locus
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Discovery year2004-04-16
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Entrez gene record
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RefSeq identity
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Classification
- Fibronectin type III domain containing
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namefibronectin leucine rich transmembrane protein 3
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GenBank acession
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Locus
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Discovery year1999-10-29
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Fibronectin type III domain containing
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VEGA ID
MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data