ARC Antibody Apoptosis Repressor with CARD

• Catalog number
  R30891
• Price
  Please ask
• Size
  0.1mg

• Category
  Antibody
• Concentration
  0.5mg/ml if reconstituted with 0.2ml sterile DI water
• Form
  Antigen affinity purified
• Conjugation
  Unconjugated
• Clone
  Polyclonal antibody
• Recognised antigen
  ARC Apoptosis Repressor with CARD
• Host animal
  Rabbit (Oryctolagus cuniculus)
• Clonality
  Polyclonal (rabbit origin)
• Species reactivity
  Human (Homo sapiens), Mouse (Mus musculus), Rat ; Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur.
• Tested applications
  WB
• Recommended dilutions
  Western blot: 0.5-1ug/ml
• Notes
  The stated application concentrations are suggested starting amounts. Titration of the ARC antibody may be required due to differences in protocols and secondary/substrate sensitivity.
• Intented use
  This ARC antibodyis to be used only for research purposes and not for diagnostics..
• Uniprot
  O60936
• Purity
  Antigen affinity
• Description
  Apoptosis Repressor with CARD, also known as ARC, NOP30, NOL3, CARD2 and MYP, is a protein that in humans is encoded by the NOL3 gene. NOL3 has been shown to interact with SFRS9 and Caspase 8. By genomic sequence analysis, Stoss et al.(1999) determined that the NOL3 gene, which encodes NOP30 and MYP and which they called NOP, is composed of 4 exons. The alternative 5-prime splice site that generates the two isoforms is located in exon 2. It is reported that expression of the Apoptosis Repressor with CARD/ARC cDNA encoding the smaller transcript inhibited apoptosis in a dose-dependent manner when coexpressed with CASP8 but not when coexpressed with CASP9. The protein also inhibited apoptosis induced by stimulation of CD95/FAS, tumor necrosis factor receptor-1, and TRAMP/death receptor-3. Enzymatic analysis showed that ARC inhibits the enzymatic activity of CASP8. Immunoprecipitation and immunoblot analysis indicated that ARC interacts with CASP2 and -8 through its N-terminal death effector domain but does not interact with CASP1, -3, or -9.
• Immunogen
  Amino acids 91-106 (WDWQHVGPGYRDRSYD-human) were used as the immunogen for this ARC antibody (100% rat homology).
• Storage
  After reconstitution, the ARC antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20 deg. Celcius or lower. Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity. Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized. Avoid repeated freezing and thawing.
• Properties
  If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
• Additional description
  This 1 is suited for programmed cell-death studies.
• French translation
  anticorps

• Gene target
  ARC   Repressor   with   CARD  
• Gene symbol
  ARPC1B, ARC, ARPC3, ARPC4, NOL3, ARPC2, ARPC5
• Short name
  Anti-ARC Apoptosis Repressor with CARD
• Technique
  apoptosis, Antibody, antibodies against human proteins, antibodies for, apoptosis pathway
• Isotype
  Rabbit IgG
• Alternative name
  Antibodies to ARC Apoptosis Repressor with CARD
• Alternative technique
  celldeath, antibodies
• Alternative to gene target
  activity-regulated cytoskeleton-associated protein, Arg3.1, ARC and IDBG-37634 and ENSG00000198576 and 23237, protein binding, Cell surfaces, Arc and IDBG-149967 and ENSMUSG00000022602 and 11838, ARC and IDBG-648018 and ENSBTAG00000021639 and 519403

Gene info

• Identity
  HGNC:704
• Gene
  ARPC1B
• Long gene name
  actin related protein 2/3 complex subunit 1B
• Synonyms gene name
  • actin related protein 2/3 complex, subunit 1B (41 kD)
  • actin related protein 2/3 complex subunit 1B, 41kDa
• Synonyms
  • ARC41
  • p40-ARC
  • p41-ARC
• Synonyms name
  • ARP2/3 protein complex subunit p41
  • actin related protein 2/3 complex, subunit 1A (41 kD)
• GenBank acession
  • AF006084
• Locus
  7q22.1
• Discovery year
  1999-08-06
• Entrez gene record
  10095
• Pubmed identfication
  • 9230079
  • 9359840
• RefSeq identity
  • NM_005720
• Classification
  • WD repeat domain containing
  • Actin related protein 2/3 complex subunits
• VEGA ID
  OTTHUMG00000154552
• Locus Specific Databases
  • LRG_1188

Gene info

• Identity
  HGNC:648
• Gene
  ARC
• Long gene name
  activity regulated cytoskeleton associated protein
• Synonyms
  • KIAA0278
  • Arg3.1
• GenBank acession
  • AF193421
• Locus
  8q24.3
• Discovery year
  2000-05-23
• Entrez gene record
  23237
• Pubmed identfication
  • 10970730
  • 17466953
• Classification
  • Gag like LTR retrotransposon derived genes
• VEGA ID
  OTTHUMG00000134310

Gene info

• Identity
  HGNC:706
• Gene
  ARPC3
• Long gene name
  actin related protein 2/3 complex subunit 3
• Synonyms gene name
  • actin related protein 2/3 complex, subunit 3 (21 kD)
  • actin related protein 2/3 complex subunit 3, 21kDa
• Synonyms
  • p21-Arc
  • ARC21
• GenBank acession
  • AF006086
• Locus
  12q24.11
• Discovery year
  1999-08-06
• Entrez gene record
  10094
• Pubmed identfication
  • 9230079
  • 9359840
• Classification
  • Actin related protein 2/3 complex subunits
• VEGA ID
  OTTHUMG00000134333

Gene info

• Identity
  HGNC:707
• Gene
  ARPC4
• Long gene name
  actin related protein 2/3 complex subunit 4
• Synonyms gene name
  • actin related protein 2/3 complex, subunit 4 (20 kD)
  • actin related protein 2/3 complex subunit 4, 20kDa
• Synonyms
  • p20-Arc
  • ARC20
• Synonyms name
  • Arp2/3 protein complex subunit p20
  • actin related protein 2/3 complex, subunit 4 (20 kD)
• GenBank acession
  • AF019888
• Locus
  3p25.3
• Discovery year
  1999-08-06
• Entrez gene record
  10093
• Pubmed identfication
  • 9230079
  • 9359840
• RefSeq identity
  • NM_001024959
• Classification
  • Actin related protein 2/3 complex subunits
• VEGA ID
  OTTHUMG00000133768

Gene info

• Identity
  HGNC:7869
• Gene
  NOL3
• Long gene name
  nucleolar protein 3
• Synonyms gene name
  • nucleolar protein 3 (apoptosis repressor with CARD domain)
• Synonyms
  • ARC
  • NOP30
  • MYP
  • CARD2
• GenBank acession
  • AF043244
• Locus
  16q22.1
• Discovery year
  1999-01-13
• Entrez gene record
  8996
• Pubmed identfication
  • 9560245
• Classification
  • Caspase recruitment domain containing
• VEGA ID
  OTTHUMG00000173316

Gene info

• Identity
  HGNC:705
• Gene
  ARPC2
• Long gene name
  actin related protein 2/3 complex subunit 2
• Synonyms gene name
  • actin related protein 2/3 complex, subunit 2 (34 kD)
  • actin related protein 2/3 complex subunit 2, 34kDa
• Synonyms
  • p34-Arc
  • ARC34
• GenBank acession
  • AF006085
• Locus
  2q35
• Discovery year
  1999-08-06
• Entrez gene record
  10109
• Pubmed identfication
  • 9359840
  • 9230079
• RefSeq identity
  • NM_005731
• Classification
  • Actin related protein 2/3 complex subunits
• VEGA ID
  OTTHUMG00000133618

Gene info

• Identity
  HGNC:708
• Gene
  ARPC5
• Long gene name
  actin related protein 2/3 complex subunit 5
• Synonyms gene name
  • actin related protein 2/3 complex, subunit 5 (16 kD)
  • actin related protein 2/3 complex subunit 5, 16kDa
• Synonyms
  • p16-Arc
  • ARC16
  • dJ127C7.3
• Synonyms name
  • Arp2/3 protein complex subunit p16
• GenBank acession
  • AF017807
• Locus
  1q25.3
• Discovery year
  1999-08-06
• Entrez gene record
  10092
• Pubmed identfication
  • 9359840
  • 9230079
• RefSeq identity
  • NM_005717
• Classification
  • Actin related protein 2/3 complex subunits
• VEGA ID
  OTTHUMG00000035326

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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