ELISA test for Mouse Biotin--protein ligase (HLCS)

• Catalog number
  AE39912MO-48
• Price
  Please ask
• Size
  1x plate of 48 wells

• Description
  Mouse Biotin--protein ligase (HLCS) ELISA Kit is manufactured by highest quality antibodies and plates to provide you with excellent and reproducible results in your work. The specifically designed buffers will ensure optimal conditions in each step from diluting the samples, through the incubation to washing.
• Storage and shipping
  Store and ship all of of the comptents of the EIA assay for Mouse Biotin--protein ligase (HLCS) on blue ice/ice packs at +4 degrees Celcius. Afoid freezing and especially freeze-thaw cycles as such cycles may denaturate the peptide chains in the antibodies, standards and enzymes, thus reducing the activity and sensitivity of the kit.
• Advise tips
  Small volumes of the liquid components of the HLCS ELISA Kit may get caught on the vials' walls and seals. Prior to use, briefly centrifuge the vials to ensure that all of the vial's content is on the bottom of the vial.
• Properties
  Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6. E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays
• Conjugation
  Biotinylated
• Test
  ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED, Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
• Storage
  Human ELISAs or ELISA kits will be stored at +4°C. The expiry date is mostly determined by the standard stability. If your ELISA kit is expired we can supply a new standard so it will still be functional.
• Group
  Enzyme-Linked Immunosorbent Assay kits
• Latin name
  Mus musculus

• Gene target
  for   protein   ligase   HLCS  
• Gene symbol
  HLCS-AS1, HLCS-IT1, HLCS
• Short name
  HLCS ELISA Kit
• Technique
  ELISA test, ELISA, Mouse, ELISAs Enzyme-linked immunosorbent assay 90320007 SNOMED code are used by the medical researcher for detection of human, mouse, … proteins are supplied in coated 96 well plates to be stored at +4°C. ELISA test kits can be sandwich ELISA. In sandwich ELISAS Enzyme-linked immunosorbent assay 9we use captor and detector antibodies. In single antibody ELISAs the antigen is coated and only a detector antibody is used. Traditional competition antigen ELISAs are coated with a captor antibody and a competitive antigen is labelled with the chromogen. In this case the ratio on your graph will be inverse proportional with the antigen present in your sample. A sign detected by the Colorimetric detection OD optical density absorbance at 450 nm between 0 and 4 with an ELISA reader for 450 nm absortion. Genprice Inc. the recognized authority tax ID 45-4304622 D-U-N-S number - 078440800. MeSH code E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay. PROMT code 3841327 Immunoabsorbent assay tests. mouses
• Host
  mouse, antigen assay ELISA kit 1 Kit (96 Wells)
• Label
  Biotin
• Species
  Mouse (Mus musculus), Mouses
• Alternative name
  EIA assay for Mouse Biotin--protein ligase (HLCS)
• Alternative technique
  murine, elisas, tests

Gene info

• Identity
  HGNC:55850
• Gene
  HLCS-AS1
• Long gene name
  HLCS antisense RNA 1
• Locus
  21q22.13
• Discovery year
  2021-09-01
• Classification
  • Antisense RNAs

Gene info

• Identity
  HGNC:41343
• Gene
  HLCS-IT1
• Long gene name
  HLCS intronic transcript 1
• Synonyms gene name
  • HLCS intronic transcript 1 (non-protein coding)
• Locus
  21q22.13
• Discovery year
  2011-05-19
• Classification
  • Intronic transcripts
• VEGA ID
  OTTHUMG00000086640

Gene info

• Identity
  HGNC:4976
• Gene
  HLCS
• Long gene name
  holocarboxylase synthetase
• Synonyms gene name
  • holocarboxylase synthetase (biotin-[proprionyl-Coenzyme A-carboxylase (ATP-hydrolysing)] ligase)
  • holocarboxylase synthetase (biotin-(proprionyl-Coenzyme A-carboxylase (ATP-hydrolysing)) ligase)
  • holocarboxylase synthetase (biotin-(proprionyl-CoA-carboxylase (ATP-hydrolysing)) ligase)
• Synonyms
  • HCS
• Locus
  21q22.13
• Discovery year
  1994-12-15
• Entrez gene record
  3141
• Pubmed identfication
  • 7842009
• VEGA ID
  OTTHUMG00000086636

MeSH Data

• Name
  Enzyme-Linked Immunosorbent Assay
• Concept
  Scope note: An immunoassay utilizing an antibody labeled with an enzyme marker such as horseradish peroxidase. While either the enzyme or the antibody is bound to an immunosorbent substrate, they both retain their biologic activity; the change in enzyme activity as a result of the enzyme-antibody-antigen reaction is proportional to the concentration of the antigen and can be measured spectrophotometrically or with the naked eye. Many variations of the method have been developed.
• Tree numbers
  • E05.478.566.350.170
  • E05.478.566.380.360
  • E05.478.583.400.170
  • E05.601.470.350.170
  • E05.601.470.380.360
• Qualifiers
  ethics, mortality, psychology, trends, veterinary, history, classification, economics, instrumentation, methods, nursing, standards, adverse effects, statistics & numerical data

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