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Clone
6G7-2H5
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Immunogen
Raised against a synthetic peptide of human GRP170
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Antibody s full description
Mouse Anti-Human GRP170 Monoclonal IgG2a Antibody, Clone: 6G7-2H5: Unconjugated
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Antibody s category
Monoclonal Antibodies
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Antibody s other name
ORP150 Antibody, HSP12A Antibody, Hypoxia up regulated 1 Antibody, Orp150 Antibody, Glucose regulated Antibody 170 Antibody, 150kDa oxygen regulated Antibody Antibody
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Raised in
Mouse
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Antibody s target
GRP170
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Primary research fields
Cancer, Heat Shock, Neuroscience, Neurodegeneration, Epigenetics, Trafficking, cardiovascular System
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Brandname
None
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Antibodies applications
WB, ICC/IF
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Antibody s reactivity
Human
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Antibody s dilutions
WB (1:1000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
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Purity
Protein G Purified
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Antibody buffer for storage
PBS pH7.4, 50% glycerol, 0.1% sodium azide
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Antibody s concentration
1 mg/ml
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Antibody s specificity
Detects ~170kDa.
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Storage recommendations
-20ºC
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Shipping recommendations
Blue Ice or 4ºC
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Antibody certificate of analysis
1 µg/ml of SMC-233 was sufficient for detection of GRP170 in 20 µg of HEK293 lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
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Antibody in cell
Endoplasmic Reticulum , Endoplasmic Reticulum Lumen
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Tissue specificity
See included datasheet or contact our support service
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Scientific context
GRP170, also known as ORP150, is the largest member of glucose-regulated Antibodys, and acts as a human chaperone Antibody. It is thought to play an important role in Antibody folding and secretion in the ER. Suppression of the Antibody is associated with accelerated apoptosis, therefore having an important cryoprotective role in hypoxia-induced cellular pertubation. This cryopotective role has led to an anti-tumor immune response, which will hopefully lead to therapeutic immunizations against cancers (1). GRP170 has also been shown to bind with dendritic cells and provide the danger signals to induce anti-tumor immune responses (2).
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Bibliography
1. Wang H., et al. (2014) Front Oncol. 4: 377. 2. Manjili M.H., et al. (2006) Immun. Cell Biol. 84: 203-208.
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Released date
2-Jul-2015
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Tested applications
To be tested
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Tested reactivity
To be tested
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NCBI number
NP_006389.3
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Gene number
10525
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Protein number
Q9Y4L1
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Antibody s datasheet
Contact our support service
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Representative figure link
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Representative figure legend
Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-GRP170 Monoclonal Antibody, Clone 6G7-2H5 (SMC-233). Tissue: Neuroblastoma cell line SK-N-BE. Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-GRP170 Monoclonal Antibody (SMC-233) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cytoplasm, Endoplasmic Reticulum. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) GRP170 Antibody (D) Composite. Western Blot analysis of Human HEK-293 cell lysate showing detection of ~170 kDa GRP170 protein using Mouse Anti-GRP170 Monoclonal Antibody, Clone 6G7-2H5 (SMC-233). Lane 1: Molecular Weight Ladder (MW). Lane 2: HEK-293 cell lysate. Load: 20 µg. Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-GRP170 Monoclonal Antibody (SMC-233) at 1:1000 for 16 hours at 4°C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:100 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~170 kDa. Mouse Anti-GRP170 Antibody [6G7-2H5] used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human Neuroblastoma cell line SK-N-BE (SMC-233) Mouse Anti-GRP170 Antibody [6G7-2H5] used in Western Blot (WB) on Human HEK-293 cell lysate (SMC-233)
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Warning information
Non-hazardous
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Country of production
Canada
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Total weight kg
1.4
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Net weight g
0.1
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Stock availability
In Stock
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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Test
StressMark antibodies supplies antibodies that are for research of human proteins. Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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About
Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
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Latin name
Mus musculus
