His-Tag Antibody
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Catalog number3998-100
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PricePlease ask
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Size100 µg
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Antibody TargetHis-Tag
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Target Alternative NameHis tag, HHHHHH tag
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Antibody TypePolyclonal
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Species ReactivitiesAll species
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Immunogen Sequence6xHis synthetic peptide
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AppearanceColorless liquid
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FormLiquid
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Concentration0.5 mg/ml
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Formulation100 µg (0.5 mg/ml) peptide affinity purified rabbit anti-His-Tag polyclonal antibody in phosphate buffered saline (PBS, pH 7.2), containing 50% glycerol, 1% BSA, 0.02% thimerosal.
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PurificationAffinity purified
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ApplicationWestern blot, Immunoprecipitation/IC
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Application And UsagesWestern blotting (1 µg/ml), immunoprecipitation (40 µg/ml), and immunocytochemistry (2 µg/ml). However, the optimal conditions should be determined individually. The antibody detects His-tagged recombinant proteins.
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DescriptionA variety of plasmids contain DNA that encodes an N-terminal tag consisting of six histidine (His) residues, followed by an extended multiple cloning sites. The 6xHis tag on the expressed recombinant proteins allows for efficient coupling to Ni++ affinity resins and purification by a single step chromatography. As is the case with other protein tags, this polyhistidine tag can be cleaved at sites recognized by enzymes such as thrombin and enterokinases to isolate the protein of interest.
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Storage Condition-20 °C
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Shipping Conditiongel pack
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Shelf Life12 months
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UsageFor Research Use Only! Not to be used in humans.
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HandlingThe antibody solution should be gently mixed before use.
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Western Blot VerifiedVerified
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Immunocytochemistry VerifiedNot Verified
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Immunofluorescence VerifiedNot Verified
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Immunoprecipitation VerifiedNot Verified
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FACS VerifiedNot Verified
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ELISA VerifiedNot Verified
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ChIP VerifiedNot Verified
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Flow Cytometry VerifiedNot Verified
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PropertiesIf you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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Conjugationhistidine
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French translationanticorps
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Gene target
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Short nameHis-Tag Antibody
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TechniqueAntibody, antibodies against human proteins, antibodies for, Tags
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IsotypeRabbit IgG
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LabelHis
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Alternative namehistidine-detection labelled (antibody to-)
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Alternative techniqueantibodies
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MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data