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Synonyms
RANKL, CD254, TRANCE, OPGL, ODF
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Alternative_names
RANKL, CD254, TRANCE, OPGL, ODF
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Description
Tumor necrosis factor ligand superfamily member 11 stimulates osteoclast formation.
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Recombinant
Yes
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Source
HEK293 cells
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Purity by SDS PAGE
> 95%
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Assay
SDS-PAGE
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Endotoxin Level
< 1.0 EU per/ug
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Activity Specifications test method
Measured by its binding ability in a functional ELISA.
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Biological activity
Immobilized Human TNFRSF11A at 5 ug/mL (100 uL/well) can bind Human TNFSF11, His Tag with a linear range of 0.2-6 ng/mL
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Binding Capacity
Immobilized Human TNFRSF11A at 5 ug/mL (100 uL/well) can bind Human TNFSF11, His Tag with a linear range of 0.2-6 ng/mL
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Molecular Weight
23.1 kDa
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Storage Temp
-20°C
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Shipping
Gel Pack
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Shelf Life
12 months
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Appearance
Lyophilized
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Physical form description
Lyophilized from 0.22 um filtered solution in PBS, pH7.4. Generally Mannitol or Trehalose is added as a protectant before lyophilization.
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Reconstitution Instructions
Reconstitute in sterile deionized water to the desired protein concentration.
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Background Information
Receptor activator of nuclear factor kappa-B ligand (RANKL), also known as tumor necrosis factor ligand superfamily member 11 (TNFSF11), TNF-related activation-induced cytokine (TRANCE), osteoprotegerin ligand (OPGL), and osteoclast differentiation factor (ODF), is known as a type II membrane protein and is a member of the tumor necrosis factor (TNF) superfamily. RANKL, through its ability to stimulate osteoclast formation and activity, is a critical mediator of bone resorption and overall bone density. Some findings also suggestion some cancer cells, particularly prostate cancer cells, can activate an increase in bone remodeling and ultimately increase overall bone production.[17] This increase in bone remodeling and bone production increases the overall growth of bone metastasizes.
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Handling
Centrifuge the vial prior to opening.
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Usage
For Research Use Only! Not to be used in humans
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Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Additional source
Recombinants or rec. proteins
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Group
recombinants
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Gene target
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Gene symbol
TNFSF11
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Short name
CellExp™ TNFSF11 / RANKL / CD254, Recombinant
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Technique
Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
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Species
Humans
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Alternative name
H. sapiens CellExp™ tumor necrosis factor (ligand) superfamily, member 11 / RANKL / CD254, H. sapiens Rec.
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Alternative technique
rec
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Alternative to gene target
tumor necrosis factor (ligand) superfamily, member 11, CD254 and hRANKL2 and ODF and OPGL and OPTB2 and RANKL and sOdf and TRANCE, TNFSF11 and IDBG-28393 and ENSG00000120659 and 8600, tumor necrosis factor receptor superfamily binding, Extracellular, Tnfsf11 and IDBG-182101 and ENSMUSG00000022015 and 21943, TNFSF11 and IDBG-628731 and ENSBTAG00000008924 and 513836
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