Enteropeptidase/Enterokinase Cleavage Kit

Summary

Sufficient for cleaving at least 5 mg of target protein
Features Benefits

• Efficiently removes tags from recombinant fusion proteins containing accessible Enteropeptidase-specific recognition sequence. • Includes cleavage control protein (hPRL-Trx)
Storage Conditions

-20°C
Shipping Conditions

gel pack
Shelf life

12 months
Background

Enteropeptidase (Enterokinase, EC 3.4.21.9) is a serine protease involved in activation of trypsinogen to trypsin, which in turn results in the activation of various digestive enzymes. It recognizes a highly specific amino acid sequence ‘DDDDK’ and cleaves after the lysine residue (K). The high specific activity of Enteropeptidase has been utilized in cleaving a variety of native or fusion proteins containing the above recognition motif. Biovision’s Enteropeptidase Cleavage Kit contains highly active light chain fragment of human Enteropeptidase. Our pure enzyme is an excellent tool to obtain a wild type protein sequence from a fusion protein, containing Enteropeptidase recognition sequence (DDDDK). This Enteropeptidase cleavage kit is sufficient for cleaving at least 5 mg of target protein. The residual enteropeptidase left in the reaction mix will not interfere with most of the downstream applications. Following cleavage of the target protein, Enteropeptidase can be removed using BioVision Hi-Bind Ni-QR Agarose beads.
Data Sheet Link

Gene target

Enteropeptidase/Enterokinase Cleavage Kit
Short name

Enteropeptidase/Enterokinase Cleavage Kit
Alternative name

Enteropeptidase/Enterokinase Cleavage reagent
Alternative technique

kits
Alternative to gene target

v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832