-
Synonyms
Single chain Urokinase-type plasminogen activator (scuPA), Urokinase-type Plasminogen Activator uPA, PLAU.
-
Alternative_names
Single chain Urokinase-type plasminogen activator (scuPA), Urokinase-type Plasminogen Activator uPA, PLAU.
-
Description
After activation, forms Urokinase, which is a serine protease
-
Recombinant
Yes
-
Source
E. coli.
-
Purity by SDS PAGE
≥90%
-
Assay
SDS-PAGE
-
Activity Specifications test method
The enzyme is fully active as seen from its ability to cleave a fluorogenic substrate N-carbobenzyloxy-Gly-Gly-Arg-7-amido-4-methylcoumarin (Z-GGR-AMC) (Cat # K728-100).
-
Biological activity
>1200 mU/mg (1 U = Digestion of 1 µmole of Z-GGR-AMC substrate in 1 min at 37ºC.)
-
Results
>1200 mU/mg (1 U = Digestion of 1 µmole of Z-GGR-AMC substrate in 1 min at 37ºC.)
-
Unit Definition
1 U = Digestion of 1 µmole of Z-GGR-AMC substrate in 1 min at 37ºC.
-
Molecular Weight
49.3 kDa
-
Storage Temp
-20°C
-
Shipping
gel pack
-
Shelf Life
12 months
-
Appearance
Lyophilized powder
-
Physical form description
Lyophilized from proprietary buffer.
-
Reconstitution Instructions
Briefly spin down the vial and reconstitute in water to 0.5-1 mg/ml and store at –80°C.
-
Background Information
Urokinase or Urokinase-type plasminogen activator (uPA) is a serine protease (EC 3.4.21.73). It is secreted as a single-chain zymogen, pro-Urokinase, possessing little or no intrinsic enzymatic activity. The single chain zymogen is converted into the active two chain enzyme (tcuPA) by cleavage of the bond between Lys157 and Ile158. After activation, Urokinase specifically cleaves the proenzyme plasminogen to form the active enzyme plasmin. The active plasmin then catalyzes the breakdown of fibrin polymers of blood clots. Urokinase is involved in a number of biological functions including fibrinolysis, embryogenesis, cell migration, tissue remodeling, ovulation, and wound healing. Additionally, it is a potent marker of invasion and metastasis in a variety of human cancers associated with breast, stomach, colon, bladder, ovary, brain and endometrium.
-
Handling
Centrifuge the vial prior to opening.
-
Usage
For Research Use Only! Not to be used in humans
-
-
Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
-
Additional source
Recombinants or rec. proteins
-
Group
recombinants