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Synonyms
ERBB2, CD340, HER-2/neu, HER2, MLN19, NEU, NGL, TKR1
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Alternative_names
ERBB2, CD340, HER-2/neu, HER2, MLN19, NEU, NGL, TKR1
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Description
Involved in the signal transduction pathways leading to cell growth and differentiation.
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Recombinant
Yes
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Source
HEK293 cells
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Purity by SDS PAGE
≥95%
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Assay
SDS-PAGE
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Activity Specifications test method
Measured by its binding ability in a functional ELISA.
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Biological activity
Immobilized Trastuzumab at 2 ug/mL (100 uL/well) can bind Cynomolgus Her2 with a linear range of 2-6 ng/mL
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Binding Capacity
Immobilized Trastuzumab at 2 ug/mL (100 uL/well) can bind Cynomolgus Her2 with a linear range of 2-6 ng/mL
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Molecular Weight
71.2 kDa
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Storage Temp
-20°C
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Shipping
Gel Pack
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Shelf Life
12 months
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Appearance
Lyophilized
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Physical form description
Lyophilized from 0.22 um filtered solution in PBS, pH7.4. Generally Trehalose is added as a protectant before lyophilization.
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Reconstitution Instructions
Reconstitute in sterile deionized water to a concentration of 50 ug/ml.
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Background Information
Human Epidermal growth factor Receptor 2 (HER2) is also called ERBB2, HER-2, HER-2 /neu, NEU, NGL, TKR1 and c-erb B2, and is a protein giving higher aggressiveness in breast cancers. It is a member of the ErbB protein family, more commonly known as the epidermal growth factor receptor family. HER2 is a cell membrane surface-bound receptor tyrosine kinase and is normally involved in the signal transduction pathways leading to cell growth and differentiation. HER2 is thought to be an orphan receptor, with none of the EGF family of ligands able to activate it. Approximately 30% of breast cancers have an amplification of the HER2 gene or overexpression of its protein product. Overexpression of this receptor in breast cancer is associated with increased disease recurrence and worse prognosis. HER2 appears to play roles in development, cancer, communication at the neuromuscular junction and regulation of cell growth and differentiation.
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Handling
Centrifuge the vial prior to opening.
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Usage
For Research Use Only! Not to be used in humans
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Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Additional source
Recombinants or rec. proteins
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Group
recombinants
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Gene target
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Short name
CellExp™ Her2 / ErbB2, Cynomolgus Recombinant
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Technique
Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
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Species
Humans
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Alternative name
H. sapiens CellExp™ Her2 / v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog (avian), Cynomolgus Rec.
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Alternative technique
rec
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Alternative to gene target
v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog (avian), CD340 and HER-2 and HER-2/neu and HER2 and MLN 19 and NEU and NGL and TKR1, ERBB2 and IDBG-46068 and ENSG00000141736 and 2064, transferase activity, nuclei, Erbb2 and IDBG-210591 and ENSMUSG00000062312 and 13866, ERBB2 and IDBG-639410 and ENSBTAG00000021798 and 505709
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