-
Available ordering format
Lyophilized in PBS pH 7.4
-
Immunogen
KLH-conjugated synthetic peptide derived from Arabidopsis thaliana PIF5, UniProt: Q84LH8, TAIR: AT3G59060
-
Raised in
Rabbit
-
Clonality
Polyclonal
-
Clone
Polyclonal
-
Purification
Affinity purified serum
-
How to reconstitute
For reconstitution please add 50 µl of sterile water
-
Storage condition
store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes
-
Verified applications
western blot (WB)
-
Connected products
AS16 3156 | anti-PIF3 | Phytochrome interacting factor 3, rabbit antibodiesAS16 3157 | anti-PIF4 | Phytochrome interacting factor 4, rabbit antibodiesPlant protein extraction buffer Secondary antibodies
-
Recommended dilutions for use
1 : 1000 with ECL (WB)
-
Molecular weight expected аpparent
49.3 | 62 kDa
-
Verified reactivity
Arabidopsis thaliana
-
Possible reactivity
please inquire
-
No reactivity
Physcomitrella patens
-
Supplementary information
For detection of PIF5 please use the most sensitive detection reagent.
-
References
to be added when available, antibody released in July 2015
-
Scientific context
PIF5 (Phytochrome interacting factor 5) is a transcription factor which acts negatively in the phytochrome B signaling pathway and regulates PHYB at post-transcriptional level. Alternative protein names: AtbHLH65, bHLH65, bHLH065, Basic helix-loop-helix protein 65, PHYTOCHROME INTERACTING FACTOR 3-LIKE 6, PIL6, Phytochrome interacting factor-like 6, Transcription factor EN 103.
-
Notes
PIF proteins are not that stable, therefore special precautions should be taken during extraction and whole procedure should be performed in as little light as possible (light green light). Extraction of PIF proteins is described in Shen et al. (2007).
-
Protein number
Q84LH8
-
TAIR number
AT3G59060
-
-
Description
Aplha, transcription related growth factors and stimulating factors or repressing nuclear factors are complex subunits of proteins involved in cell differentiation. Complex subunit associated factors are involved in hybridoma growth, Eosinohils, eritroid proliferation and derived from promotor binding stimulating subunits on the DNA binding complex. NFKB 105 subunit for example is a polypetide gene enhancer of genes in B cells.
