E-64

Alternative_name

trans-Epoxysuccinyl-L-leucylamido(4-guanidino)butane, L-trans-3-Carboxyoxiran-2-carbonyl-L-leucylagmatine, N-(trans-Epoxysuccinyl)-L-leucine 4-guanidinobutylamide
Description

E-64 is an irreversible, cell-permeable, potent, and highly selective cysteine protease inhibitor. E-64 will not inhibit serine proteases (except trypsin) like other cysteine protease inhibitors, leupeptin and antipain. A very useful cysteine protease inhibitor for use in in vivo studies because it has a specific inhibition and displays low toxicity.
CAS Number

66701-25-5
Structure Available

Yes
Salt Form

No
Molecular Formula

C₁₅H₂₇N₅O₅
Molecular Weight

357.41
Cell Permeable

Yes
Purity

≥99% by HPLC
Solubilities

DMSO (25 mg/ml) or H₂O (20 mg/ml)
Handling

Protect from air and moisture
Tag Line

A cysteine protease inhibitor
Storage Condition

-20°C
Shipping Condition

RT
Shelf Life

24 months
MDL Number

MFCD00080261
PubChem CID

439487
PubChem CID Link
SMILES

CC(C)CC(C(=O)NCCCCN=C(N)N)NC(=O)C1C(O1)C(=O)O
InChi

InChI=1S/C15H27N5O5/c1-8(2)7-9(20-13(22)10-11(25-10)14(23)24)12(21)18-5-3-4-6-19-15(16)17/h8-11H,3-7H2,1-2H3,(H,18,21)(H,20,22)(H,23,24)(H4,16,17,19)/t9-,10+,11+/m0/s1
InChi Key

LTLYEAJONXGNFG-HBNTYKKESA-N
Data Sheet Link
MSDS Available

Yes

Gene target

E-64
Gene symbol

IGLVIV-64, HLA-E, IGHV3-64, CSTF2, IRAK4, SMC2, SERPINA12, SNAR-E, IPCEF1
Short name

E-64
Alternative name

E-64

Identity

HGNC:5943
Gene

IGLVIV-64
Long gene name

immunoglobulin lambda variable (IV)-64 (pseudogene)
Synonyms gene name
- immunoglobulin lambda variable (IV)-64
- immunoglobulin lambda variable (IV)-64 pseudogene
Synonyms
- IGLV(IV)-64
GenBank acession
- D87022
Locus

22q11.22
Discovery year

2000-05-08
Entrez gene record

28760
RefSeq identity
- NG_000002
Classification
- Immunoglobulin lambda locus at 22q11.2
VEGA ID

OTTHUMG00000150852

Identity

HGNC:2484
Gene

CSTF2
Long gene name

cleavage stimulation factor subunit 2
Synonyms gene name
- cleavage stimulation factor, 3' pre-RNA, subunit 2, 64kD
- cleavage stimulation factor, 3' pre-RNA, subunit 2
Synonyms
- CstF-64
GenBank acession
- BC017712
Locus

Xq22.1
Discovery year

1994-12-12
Entrez gene record

1478
Pubmed identfication
- 1741396
RefSeq identity
- NM_001325
Classification
- Cleavage stimulation factor subunits
- RNA binding motif containing
VEGA ID

OTTHUMG00000022709
Locus Specific Databases
- Global Variome shared LOVD

Identity

HGNC:17967
Gene

IRAK4
Long gene name

interleukin 1 receptor associated kinase 4
Synonyms
- NY-REN-64
GenBank acession
- AF155118
Locus

12q12
Discovery year

2002-09-27
Entrez gene record

51135
Pubmed identfication
- 3772297
- 10508479
VEGA ID

OTTHUMG00000169425
Locus Specific Databases
- IRAK4base: Mutation registry for IRAK4 deficiency
- LRG_75

Identity

HGNC:14011
Gene

SMC2
Long gene name

structural maintenance of chromosomes 2
Synonyms gene
- SMC2L1
Synonyms gene name
- SMC2 (structural maintenance of chromosomes 2, yeast)-like 1
- SMC2 structural maintenance of chromosomes 2-like 1 (yeast)
Synonyms
- hCAP-E
- CAP-E
GenBank acession
- AF092563
Locus

9q31.1
Discovery year

2001-02-22
Entrez gene record

10592
Pubmed identfication
- 9789013
Classification
- Condensin I subunits
- Condensin II subunits
- Structural maintenance of chromosomes proteins
VEGA ID

OTTHUMG00000020401

Identity

HGNC:18359
Gene

SERPINA12
Long gene name

serpin family A member 12
Synonyms gene name
- serine (or cysteine) proteinase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 12
- serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 12
Synonyms
- OL-64
Synonyms name
- Vaspin
GenBank acession
- AY177692
Locus

14q32.13
Discovery year

2004-05-21
Entrez gene record

145264
Pubmed identfication
- 24172014
RefSeq identity
- NM_173850
Classification
- Serpin peptidase inhibitors
VEGA ID

OTTHUMG00000171349