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Immunogen
Full-length protein.
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Antibody s target
Anaplasma marginale mCherry
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Antibody s full description
Mouse Anti-mCherry Polyclonal
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Primary research fields
Tags and Cell Markers, Fluorescent Proteins
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Antibody s category
Polyclonal Antibodies
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Antibody s other name
mCherry Antibody, mcherry Antibody
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Verified applications
WB, IHC, ICC/IF, IP
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Raised in
Mouse
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Antibody s reactivity
Species Independent
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Antibody s recommended dilutions for use
WB (1:5000), IHC (1:200), ICC/IF (1:200), IP (1:100); optimal dilutions for assays should be determined by the user.
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Antibody s purified from
Peptide Affinity Purified
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Recommended buffer for storage
PBS pH 7.4, 50% glycerol, 150mM NaCl, 0.02% sodium azide
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Antibody s concentration
See included datasheet or contact our support service
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Antibody s specificity
The mCherry tag antibody can recognize mCherry and mCherry tag fusion proteins.
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Storage recommendations
-20°C
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Shipping recommendations
Blue Ice or 4°C
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Certificate of analysis
A 1:10000 dilution of SPC-1268 was sufficient for detection of mCherry in 10 µg of mCherry Recombinant Protein by ECL immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary antibody.
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Antibody in cell
Contact our support service.
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Tissue specificity
See included datasheet or contact our support service.
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Scientific context
mCherry is a fluorescent tracer in transfection and transgenic experiments. Originally it was derived from proteins isolated from Cnidarians (jelly fish, sea anemones and corals), specicially DsRed, a red fluorescent protein from the disc corals of Discosoma species. DsRed is similar in properties to GFP, but, produces a red rather than a green fluoresence. mCherry was engineered with further multiple cycles of mutation, directed modification and evolutionary selection, and in its monomeric form has an excitation maximum at 587 nm and an emission maximum at 610 nm.
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Bibliography
1. Shaner N.C., et al. (2004). Nature Biotechnology. 22(12): 1567–1572.
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Released date
6/Sep/2016
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NCBI number
Refer to NCBI
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Gene number
Refer to GenBank
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Protein number
X5DSL3
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PubMed number
Refer to PubMed
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Tested applications
To be tested
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Tested reactivity
To be tested
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Antibody s datasheet
Contact our support service to receive datasheet or other technical documentation.
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Representative figure legend
Western blot analysis of mCherry Recombinant Protein showing detection of ~28kDa mCherry protein using Mouse Anti-mCherry Polyclonal Antibody (SPC-1268). Lane 1: 1:5,000 Dilution. Lane 2: 1:10,000 Dilution. Primary Antibody: Mouse Anti-mCherry Polyclonal Antibody (SPC-1268). Predicted/Observed Size: ~28kDa. Mouse Anti-mCherry Antibody used in Western blot (WB) on mCherry Recombinant Protein (SPC-1268)
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Warning information
Non-hazardous
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Country of production
Canada
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Total weight kg
1.4
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Net weight g
0.1
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Stock availabilit
In Stock
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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Properties
This StressMark antibodies Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. StressMark antibodies FITC is highly sensitive to pH extremes.
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Conjugation
Anti-FITC Antibody
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Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin name
Mus musculus
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Group
Polyclonals and antibodies
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About
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.
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Gene target
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Short name
Mouse Anti-mCherry Polyclonal: FITC
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Technique
Polyclonal, anti-, Mouse, anti, FITC, antibody to, antibodies, Fluorescein, mouses, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
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Host
mouse
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Isotype
IgG1
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Label
FITC
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Species
Mouse, Mouses
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Alternative name
Mouse Antibody tomCherry polyclonal: fluorecein
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Alternative technique
polyclonals, murine, antibodies, fluorescine
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