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Category
Antibody
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Concentration
0.5mg/ml if reconstituted with 0.2ml sterile DI water
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Form
Antigen affinity purified
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Conjugation
Unconjugated
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Clone
Polyclonal antibody
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Recognised antigen
NFAT1
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Host animal
Rabbit (Oryctolagus cuniculus)
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Clonality
Polyclonal (rabbit origin)
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Species reactivity
Human (Homo sapiens), Mouse (Mus musculus), Rat ; Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur.
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Tested applications
WB, IHC-P
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Recommended dilutions
Western blot: 0.5-1ug/ml,IHC (Paraffin): 0.5-1ug/ml
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Notes
The stated application concentrations are suggested starting amounts. Titration of the NFAT1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
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Intented use
This NFAT1 antibodyis to be used only for research purposes and not for diagnostics..
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Uniprot
Q13469
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Purity
Antigen affinity
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Description
Nuclear factor of activated T-cells, cytoplasmic 2, also known as NFAT1 and NFATP or the preexisting component of NFAT, is present in the cytosolic fraction of unstimulated T cells and is a member of the nuclear factor of activated T cells (NFAT) family. The NFATC2 gene is mapped on 20q13.2. NFATC2 is highly homologous to NFATC1 over a limited domain which shows similarity to the Dorsal/Rel family but has a wider tissue distribution. Ectopic expression of NFATC2/NFAT1 inhibited the basal activity of the human CDK4 promoter. Additionally, both Calna-/- and Nfatc2 -/- mice had elevated protein levels of Cdk4, confirming a negative regulatory role for the calcineurin/NFAT pathway. NFAT1 controls myoblast fusion at a specific stage of myogenesis after the initial formation of a myotube and is necessary for further cell growth. Overexpression of the protein promoted differentiation of osteoclast precursor cells into tartrate-resistant acid phosphatase-positive(TRAP-positive) multinucleated osteoclast-like cells even in the absence of RANKL.
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Immunogen
An amino acid sequence from the C-terminus of human NFAT1 (KQEQNLDQTYLDDVNEIIR) was used as the immunogen for this NFAT1 antibody (100% homologous in human, mouse and rat).
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Storage
After reconstitution, the NFAT1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20 deg. Celcius or lower. Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity. Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized. Avoid repeated freezing and thawing.
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Properties
If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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French translation
anticorps
