Bim/Bod Antibody

• Catalog number
  3124-100
• Price
  Please ask
• Size
  100 µg

• Antibody Target
  Bim/Bod
• Target Alternative Name
  Bcl-2-like protein 11, BCL2L11
• Antibody Type
  Polyclonal
• Species Reactivities
  Human, mouse, rat
• Immunogen Sequence
  Synthetic peptide surrounding amino acid 25 of human Bim
• Accession
  O43521
• Gene Id
  10018
• Appearance
  Colorless liquid
• Form
  Liquid
• Concentration
  0.5 mg/ml
• Formulation
  100 µg (0.5 mg/ml) affinity purified rabbit anti-Bim/BOD polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, 0.01% thimerosal.
• Purification
  Affinity purified
• Application
  Western blot
• Application And Usages
  Western blot analysis (0.5-4 µg/ml). However, the optimal conditions should be determined individually. The antibody detects a 20 kDa Bim/BOD in samples from human, mouse and rat origins. Jurkat cell lysate can be used as a positive control.
• Description
  Members of the Bcl-2 family proteins all contain Bcl-2 homology domains including BH1, BH2, BH3, and BH4. The BH3 domain is known to be important for the cell killing activity of Bcl-2 members including Bad, Bax, Bid, Bik, and the recently identified BH3 domain containing protein designated Bim/BOD. Bim/BOD interacts with diverse members of the Bcl-2 sub-family including Bcl-2, Bcl-x, and Bcl-w, to induce apoptosis. There are three splice variants: BimL, BimS, and BimEL, all of which share homology in their C-terminal BH3 domains. Of the isoforms, BimS is the most potent inducer of apoptosis. The mRNA of Bim is ubiquitously expressed in multiple tissues and cell lines.
• Storage Condition
  -20 °C
• Shipping Condition
  gel pack
• Shelf Life
  12 months
• Usage
  For Research Use Only! Not to be used in humans.
• Handling
  The antibody solution should be gently mixed before use.
• Western Blot Verified
  Verified
• Immunocytochemistry Verified
  Not Verified
• Immunofluorescence Verified
  Not Verified
• Immunoprecipitation Verified
  Not Verified
• ELISA Verified
  Not Verified
• ChIP Verified
  Not Verified
• Flow Cytometry Verified
  Not Verified
• Data Sheet Link
• Properties
  If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
• French translation
  anticorps

• Gene target
  Bim/Bod  
• Gene symbol
  BCL2L11
• Short name
  Bim/Bod Antibody
• Technique
  Antibody, antibodies against human proteins, antibodies for
• Isotype
  Rabbit IgG
• Alternative name
  Bim/Bod (antibody to-)
• Alternative technique
  antibodies

Gene info

• Identity
  HGNC:994
• Gene
  BCL2L11
• Long gene name
  BCL2 like 11
• Synonyms gene name
  • BCL2-like 11 (apoptosis facilitator)
• Synonyms
  • BOD
  • BimL
  • BimEL
  • BimS
  • BIM
• GenBank acession
  • AF032458
• Locus
  2q13
• Discovery year
  1999-12-10
• Entrez gene record
  10018
• Pubmed identfication
  • 9731710
  • 9430630
• Classification
  • BCL2 homology region 3 (BH3) only
• VEGA ID
  OTTHUMG00000131256
• Locus Specific Databases
  • LRG_620

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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