Target of this antibody:
Alternative names of antibody target:
H3F3A, H3.3A, H3F3, H3F3B, H3.3B
Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Required for checkpoint-mediated arrest of cell cycle progression in response to low doses of ionizing radiation and for efficient repair of DNA double strand breaks (DSBs) specifically when modified by C-terminal phosphorylation.
This antibody reacts with:
A peptide corresponding to the C-terminus of human Histone H2AX
This antibody has been succesfully tested for use in following methods:
WB, ICC, ELISA, Multiplex
Western Blot: 0.5 ug/mL – 2 ug/mL; ICC: 1 ug/mL - 2 ug/mL; ELISA: 0.2 ug/mL - 1 ug/mL; Multiplex: 0.2 ug/mL – 1 ug/mL.
Shelf time: 12 months in temp.: -20 deg.C
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.Rabbits are used for polyclonal antibody production by Biovision. Rabbit antibodies are very stable and can be stored for several days at room temperature. Biovision adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
anti-Anti-Histone H2AX Rabbit Monoclonal Antibody
Antibody, Rabbit, anti
antibody to-Anti-Histone H2AX production species: rabbit monoclonal (Antibody to)
Long gene name H2A.X variant histone
Synonyms gene name
- H2A histone family, member X
- H2A histone family member X
Discovery year 1994-11-22
Entrez gene record 3014
Havana BLAST/BLAT OTTHUMG00000166196