Anti- Histone H3.3 Rabbit Monoclonal Antibody
#
-
Catalog numberA1140-25
-
Price:
-
Size25 µg
-
-
Antibody TargetH3F3A
-
Target Alternative NameH3F3A, H3.3A, H3F3, H3F3B, H3.3B
-
Antibody TypeMonoclonal
-
CloneRM190
-
Species ReactivitiesHuman
-
Immunogen SequenceA peptide corresponding to human Histone H3.3.
-
AccessionP84243
-
Gene Id3021
-
AppearanceColorless liquid
-
FormLiquid
-
Concentration1 mg/ml
-
FormulationIn 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
-
PurificationAffinity purified using protein A
-
ApplicationWB, ELISA, Multiplex
-
Positive ControlWB: Hela cell lysate
-
Application And UsagesWestern Blot: 0.5 ug/mL – 2 ug/mL; ELISA: 0.2 ug/mL - 1 ug/mL; Multiplex: 0.05 ug/mL – 0.5 ug/mL.
-
DescriptionVariant histone H3 which replaces conventional H3 in a wide range of nucleosomes in active genes. Constitutes the predominant form of histone H3 in non-dividing cells and is incorporated into chromatin independently of DNA synthesis. Deposited at sites of nucleosomal displacement throughout transcribed genes, suggesting that it represents an epigenetic imprint of transcriptionally active chromatin. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
-
Storage Condition-20 °C
-
Shipping Conditiongel pack
-
Shelf Life12 months
-
UsageFor Research Use Only! Not to be used in humans.
-
HandlingThe antibody solution should be gently mixed before use.
-
Western Blot VerifiedVerified
-
Immunocytochemistry VerifiedNot Verified
-
Immunofluorescence VerifiedNot Verified
-
Immunoprecipitation VerifiedNot Verified
-
FACS VerifiedNot Verified
-
ELISA VerifiedNot Verified
-
ChIP VerifiedNot Verified
-
Dot Blot VerifiedNot Verified
-
Flow Cytometry VerifiedNot Verified
-
Additional descriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
-
PropertiesIf you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
-
AboutMonoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes. Rabbits are used for polyclonal antibody production by Biovision. Rabbit antibodies are very stable and can be stored for several days at room temperature. Biovision adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
-
Latin nameOryctolagus cuniculus
-
French translationanticorps
-
Gene target
-
Short nameAnti- Histone H3 3 Rabbit Monoclonal Antibody
-
TechniqueAntibody, Rabbit, anti, antibody to, antibodies against human proteins, antibodies for, Monoclonals or monoclonal antibodies
-
HostRabbit, Rabbits
-
IsotypeRabbit IgG
-
Alternative nameantibody to- Histone H3.3 production species: rabbit monoclonal (antibody to-)
-
Alternative techniqueantibodies, rabbit-anti
-
MeSH Data
-
Name
-
ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
-
Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
-
Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data