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Immunogen
A phospho-specific peptide corresponding to residues surrounding Ser677 of human TTK (AA674-680)
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Antibody s target
Human TTK (pSer677)
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Antibody s full description
Rabbit Anti-Human TTK (pSer677) Polyclonal
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Antibody s category
Polyclonal Antibodies
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Antibody s other name
Dual specificity protein kinase TTK Antibody, ECSTY kinase Antibody, ESK Antibody, FLJ38280 Antibody, hMPS1 Antibody, Monopolar Spindle 1 Like 1 Antibody, MPH1 Antibody, Mps 1 Antibody, MPS1L1 Antibody, Phosphotyrosine Picked Threonine Kinase Antibody, Phosphotyrosine picked threonine-protein kinase Antibody, PYT Antibody, RP3-357D13.2 Antibody, ttk Antibody, TTK Protein Kinase Antibody, TTK_HUMAN Antibody, cancer/testis antigen 96 Antibody, CT96 Antibody
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Verified applications
WB, AM
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Raised in
Rabbit
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Antibody s reactivity
Human
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Antibody s recommended dilutions for use
WB (1:250); optimal dilutions for assays should be determined by the user.
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Antibody s purified from
Peptide Affinity Purified
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Recommended buffer for storage
PBS pH7.4, 50% glycerol, 0.025% Thimerosal
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Antibody s concentration
See included datasheet or contact our support service
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Antibody s specificity
Detects 97.072 kDa.
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Storage recommendations
-20°C
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Shipping recommendations
Blue Ice or 4°C
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Certificate of analysis
A 1:250 dilution of SPC-1091 was sufficient for detection of TTK (pSer677) in 10 µg of HeLa cell lysate by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
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Antibody in cell
Membrane, Cytoskeleton, Spindle
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Tissue specificity
Expressed in rapidly proliferating cell lines.
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Scientific context
TTK is a dual-specificity protein kinase with activity toward serine, threonine and tyrosine residues. TTK was found to have a role in cell cycle progression through regulating centrosome duplication during S-phase. This function was CDK2-mediated by direct activation of TTK. Overexpression or dysregulation of TTK activtiy may promote cancer through aberrant duplication of centrosomes and promotiong of aneuploidy. Mutations in TTK are highly correlated in the development of microsatellite-unstable colorectal cancers. These mutations were typically loss of function frameshift truncation, however the functional relevance of the mutation was still not determined.
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Bibliography
Refer to PubMed
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Released date
29/Aug/2016
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NCBI number
NP_003309
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Gene number
7272
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Protein number
P33981
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PubMed number
Refer to PubMed
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Tested applications
To be tested
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Tested reactivity
To be tested
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Antibody s datasheet
Contact our support service to receive datasheet or other technical documentation.
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Representative figure link
No Data Available
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Warning information
Non-hazardous
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Country of production
Canada
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Total weight kg
1.4
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Net weight g
0.1
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Stock availabilit
In Stock
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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Test
StressMark antibodies supplies antibodies that are for research of human proteins.
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Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Group
Polyclonals and antibodies
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About
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera. Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
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Latin name
Oryctolagus cuniculus