Acetoacetate Colorimetric Assay Kit

• Catalog number
  K650-100
• Price
  Please ask
• Size
  100 assays

• Description
  Only kit on the market to measure acetoacetate
• Summary
  • Detection method- Absorbance (550 nm) • Applications: - Measurement of acetoacetate in various biological samples • Analysis of acetoacetate in pathological conditions such as Type I diabetes, alcoholism, etc.
• Detection Method
  Absorbance (550 nm)
• Applications
  • Measurement of acetoacetate in various biological samples. • Analysis of acetoacetate in pathological conditions such as Type I diabetes, alcoholism, etc.
• Sample Type
  • Serum, plasma, blood, urine & other body fluids
• Features Benefits
  • Specific for acetoacetate and does not detect 3-β-hydroxybutyrate • The assay can detect less than 25 µM of acetoacetate in various sample types.
• Storage Conditions
  -20°C
• Shipping Conditions
  gel pack
• Shelf life
  12 months
• Background
  Acetoacetate (AcAc), a β-ketoacid, is one of the three ketone bodies and is formed via condensation of two molecules of acetyl-CoA in liver mitochondria. AcAc can be enzymatically reduced to 3-β-hydroxybutyrate (β-HB), or decarboxylated producing acetone (CH3)2CO). Ketone bodies (βHB: 78%; AcAc: 20% & CH3)2CO: 2%) are mainly used as an alternative energy source when glucose cannot be delivered to the system. Excessive concentration of ketone bodies (ketoacidosis) is observed in patients with Type I diabetes, severe starvation or alcoholism. Traditionally, AcAc levels have been qualitatively detected using dipsticks that use sodium nitroferricyanide as a chromophore. BioVision’s Acetoacetate Assay Kit has adapted that principle with a modification that provides a sensitive method to quantitate endogenous levels of AcAc in human blood, and urine. In this non-enzymatic assay, AcAc reacts with a substrate to generate a colored product that can be measured at 550 nm. The reaction is specific for AcAc and does not detect 3-β-hydroxybutyrate. The assay kit can detect samples containing acetoacetate as low as 25 µM.
• Data Sheet Link
• Test
  Biovision supplies other types of Assays as 1.
• Properties
  Colorimetric assays or detection use UV absorption or enzymatic color reaction.

• Gene target
  Acetoacetate   Kit  
• Short name
  Acetoacetate Colorimetric Assay Kit
• Technique
  colorimetric, Assay, assays, colorimetry
• Host
  Assay
• Alternative name
  Acetoacetate colorimetry test reagent
• Alternative technique
  assays, arrays, kits
• Alternative to gene target
  v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832

Product images

Similar products