anti-TECK Antibody [1.2_4G1-1G4-1C9]
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Catalog number
XP-5725-M
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Price
Please ask
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Size
0.5 mg
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Gene info
This gene belongs to the subfamily of small cytokine CC genes. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The CC cytokines are proteins characterized by two adjacent cysteines. TECK displays chemotactic activity for dendritic cells, thymocytes, and activated macrophages but is inactive on peripheral blood lymphocytes and neutrophils. TECK binds to chemokine receptor CCR9. Two alternatively spliced variants encoding two different isoforms have been identified.
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Gene names
Thymus expressed chemokine, Small inducible cytokine A25, CCL25, SCYA25, TECK, C-C motif chemokine 25, Chemokine TECKTECK, Ckb15
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Antibodie s label
unlabelled serum
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Purity
This IgG1k antibody was purified from ascites fluid on a Protein A affinity column.
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Ab raised in
Mouse
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Type
Monoclonal
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Monoclonal
1.2_4G1-1G4-1C9
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Antigen
The monoclonal antibody was produced in Balb/c X ICR F1 mice using recombinant hTECK as the immunizing antigen.
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Immuno application
ELISA, Western Blot, NELISAut
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Antibody advice
Neutralization:To yield one-half maximal inhibition [ND50] of the biological activity of hTECK (100 ng/mL), a concentration of 10.0-20.0 ug/mL of this antibody is required.ELISA:In a sandwich ELISA (assuming 100 μL/well), a concentration of 1.0-2.0 ug/mL of this antibody will detect at least 300 pg/mL of recombinant human TECK when used with our biotinylated antigen affinity purified anti-human TECK (XP-5280Bt) as the detection antibody at a concentration of approximately 0.5-1.0 ug/mL. Western Blot:To detect hTECK by Western Blot analysis this antibody can be used at a concentration of 0.25-0.50 ug/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hTECK is 0.50-1.0 ng/lane, under non-reducing conditions.
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Keep at
TECK lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20 grades C. Reconstitute in sterile water to a concentration of 0.1 - 1.0 mg/mL. The antibody is stable for at least six weeks at 2-4 grades C when reconstituted.
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Works with
Human
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Omim nr
212286066
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Ncbi nr
O15444
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Vial with antibody
Lyophilized
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NCBI GENE nr
6370
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GENE symbol
CCL25
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Antigene name
chemokine (C-C motif) ligand 25
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Source
Homo sapiens
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Protein nr
O15444
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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Properties
If you buy Antibodies supplied by proscience they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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French translation
anticorps
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Gene target
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Gene symbol
CCL25
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Short name
anti-TECK Antibody [1 2_4G1-1G4-1C9]
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Technique
Antibody, anti-, anti, antibody to, antibodies, antibodies against human proteins, antibodies for
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Alternative name
Antibody toTECK (antibody to-) [1.2_4G1-1G4-1C9]
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Alternative technique
antibodies
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Gene info
MeSH Data
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Name
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Concept
Scope note:
Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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