Actived-Caspase-3 p17 polyclonal antibody

• Catalog number
  BS7004-50ul
• Price
  Please ask
• Size
  50ul

• Alternative Name
  Caspase-3; Caspase3; CASP-3; Apopain; Cysteine protease CPP32; CPP-32; Protein Yama; SREBP cleavage activity 1; SCA-1; CASP3; CPP32
• Immunogen
  Synthetic peptide conjugated to KLH derived from within residues 150 - 200 of Human active Caspase 3.
• Swiss Prot
  P42574
• Reactivity
  Human,Mouse,Rat
• Applications
  WB IHC
• Application_all
  WB: 1:500~1:1000 IHC: 1:50~1:200
• Product
  Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Purification Purity
  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
• Storage Stability
  Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
• Specificity
  Actived-Caspase-3 p17 polyclonal antibody detects endogenous levels of Actived-Caspase-3 p17 protein.
• Usage
  Western blot (WB) analysis of Actived-Caspase-3 p17 polyclonal antibody at 1:500 dilution Lane1:Hela whole cell lysate(40ug) Lane2:Hela treated with H2O2 (100nmol/ml, 25min, phostop) whole cell lysate (40ug) Lane3:Hela treated with H2O2 (100nmol/ml, 25min, λ-ppase) whole cell lysate (40ug)
• BiowMW
  ~ 17, 35 kDa
• Note
  For research use only, not for use in diagnostic procedure.
• Properties
  If you buy Antibodies supplied by Bioworld they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
• Description
  Human and some mouse caspases are active in apoptosis and cell death and even in necrosis and inflammation. CASP Gene and orthologous enzymes have been identifies successfully in the signal transduction cascade and pathways.
• Group
  Polyclonals and antibodies
• About
  Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.
• French translation
  anticorps

• Gene target
  Actived-Caspase-3   p17  
• Short name
  Actived-Caspase-3 p17 polyclonal antibody
• Technique
  Polyclonal, Antibody, antibodies against human proteins, antibodies for, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
• Host
  Rabbit
• Alternative name
  Actived-caspase-3 p17 polyclonal (antibody to-)
• Alternative technique
  polyclonals, antibodies

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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