Acid Phosphatase / ACP1 Antibody
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Catalog number
N1079-25UG
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Price
Please ask
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Size
0.025mg
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Category
Antibody
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Concentration
0.5 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
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Form
Purified
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Conjugation
Unconjugated
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Clone
Polyclonal antibody
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Recognised antigen
Acid Phosphatase / ACP1
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Host animal
Rabbit (Oryctolagus cuniculus)
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Clonality
Polyclonal (rabbit origin)
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Species reactivity
Human (Homo sapiens) ; Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur.
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Tested applications
WB
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Recommended dilutions
Western blot: 1-3ug/ml
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Notes
Provided assay concentrations are suggestions only, ACP1 antibody titration may be required for optimal results. 1. This antibody will detect the A, C and D isoforms of Acid Phosphatase.
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Added buffer
1X Phosphate Buffered Saline (PBS), pH 7.4
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Intented use
This Acid Phosphatase / ACP1 antibodyis to be used only for research purposes and not for diagnostics..
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Gene ID
52
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Purity
Purified by protein A Affinity Chromatography
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Description
Acid phosphatases (AP) are a broad family of enzymes that differ widely in their AA length and homology, molecular weight and tissue of origin. They function to remove phosphate groups (or dephosphorylate) from proteins and perform best under acidic conditions. Disease states are sometimes characterized by abnormally high or low levels of Acid phosphatase, leading to them being used as serological and histological disease markers. Prostatic Acid Phosphatase (PAP) was initially used as a prostate cancer marker but was later replaced with Prostate Serum Antigen (PSA), a more sensitive early stage cancer marker.
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Immunogen
A recombinant protein fragment from the N-terminal portion of Acid Phosphatase / ACP1 was used as the immunogen for this antibody. This AA sequence is common to the A, C and D isoforms.
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Storage
Aliquot the ACP1 antibody and store frozen at -20 deg. Celcius or lower. Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity. Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized.
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Properties
If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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French translation
anticorps
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Gene target
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Gene symbol
ACP1, NDUFAB1
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Short name
Anti-Acid Phosphatase / ACP1
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Technique
Antibody, antibodies against human proteins, antibodies for
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Isotype
Rabbit IgG
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Alternative name
Antibodies to Acid Phosphatase / ACP1
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Alternative technique
antibodies
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Gene info
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Identity
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Gene
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Long gene name
acid phosphatase 1
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Synonyms gene name
- acid phosphatase 1, soluble
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year
1986-01-01
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Acid phosphatases
- Class II Cys-based phosphatases
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VEGA ID
Gene info
MeSH Data
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Name
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Concept
Scope note:
Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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