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Availability
Please contact us to check the availability
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Immunogen
Methylglyoxal-KLH-adduct
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Specificity
Methylglyoxal (MG)-Adducts, primarily Arg-Pyrimidine (N-(4-Carboxy-4,6-Dimethyl-5,6-Dihydroxy-1,4,5,6-Tetrahydropyrimidine-2-yl)-L-Ornithine), cross-reactivity with Tetrahydropyrimidine and 5-Hydro-5-Methylimidazolone app. 0.1% in ELISA, Immidazolysine and (Carboxyethyl)lysine
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Species reactivity
not relevant
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Product presentation
Antibody purified by ammonium sulphate from ascites, taken up in 10 mM PBS; 0.5% BSA; 0.1% NaN3
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Product category
Primary Antibody
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Antibody s raised in
Mouse
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Antibody s isotype
IgG2a
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Antibody s clone
6B
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Product is supplied as
liquid concentrate
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Recommended concentration for use
1 µg/ml
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Protein concentration
100 µg/ml
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Positive control
nephropathic renal tissue
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Tested applications
IHC(C, P), WB
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Recommended secondary reagent
We recommend the use of Gentaur's Universal Staining System DAB (Art. No. DA005) or AEC (Art. No. AE005), if higher sensitivity is required VECTASTAIN Elite ABC Mouse IgG (Art. No. PK-6102) or ABC AP Mouse IgG (Art. No. AK-5002) systems are applicable.
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Storage method
Store at -20°C
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UniProt number
Go to UniProt/SwissProt
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Litterature
1. Oya T., Hattori N., Mizuno Y., Miyata S., Maeda S., Osawa T., and K. Uchida (1999) Methylglyoxal modification of protein. Chemical and immunochemical characterization of methylglyoxal-arginine adducts. J. Biol. Chem. 274(26); 18492-18502. 2. Van Herreweghe F., Mao J., Chaplen F.W.R., Grooten J., Gevaert K., Vandekerckhove J., and K. Vancompernolle (2002) Tumor necrosis factor-induced modulation of glyoxalase 1 acticvities through phosphorylation by PKA results in cell death and is accompan 3. Shipanova I.N., Glomb M.A., and R.H. Nagaraj (1997) Protein Modification by Methylglyoxal: Chemical nature and synthetic mechanism of a major fluorescent adduct. Arch. Biochem. Biophys.344(1); 29-36. 4. Padayatti P.S., Ng A.S., Uchida K., Glomb M.A., and R.H. Nagaraj (2001) Argpyrimidine, a blue fluorophore in human lense proteins: high levels in brunescent cataractous lenses. Invest Ophthalmol. Vis. Sci. 42(6); 1299-1304. 5. Padayatti P.S., Jiang C., Glomb M.A., Uchida K., and R.H. Nagaraj (2001) High concentrations of glucose induce synthesis of argpyrimidine in retinal endothelial cells. Curr. Eye Res. 23(2); 106-115.
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Tips
*Gentaur antibodies are intended for in vitro research use only. They must not be used for clinical diagnostics and not for in vivo experiments in humans or animals. ** The preservative sodium azide is known to be poisonous and potentially hazardous to health. It should be handled only by trained staff. Despite of the product's low azide concentration it must be handled with care. Dispose according to regional rules!
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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About
Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
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Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin name
Mus musculus
