Caspase-9 Antibody

• Catalog number
  3136-100
• Price
  Please ask
• Size
  100 µg

• Antibody Target
  Caspase-9
• Target Alternative Name
  CASP-9 ,EC 3.4.22.62 ,ICE-like apoptotic protease 6 , ICE-LAP6,Apoptotic protease Mch-6 ,Apoptotic protease-activating factor 3 , Apaf-3
• Antibody Type
  Polyclonal
• Species Reactivities
  Human, mouse, rat
• Immunogen Sequence
  Synthetic peptide surrounding amino acid 290 of mouse caspase-9
• Accession
  BAA86895
• Gene Id
  12371
• Appearance
  Colorless liquid
• Form
  Liquid
• Concentration
  0.2 mg/ml
• Formulation
  In phosphate-buffered saline (PBS) containing 30% glycerol, 0.5% BSA, 5 mM EDTA and 0.03% proclin.
• Purification
  Affinity purified
• Application
  Western blot
• Application And Usages
  Western blotting (0.5-4 µg/ml). However, the optimal conditions should be determined individually. The antibody detects 45 kDa procaspase-9, 35/37 kDa cleaved immediate fragments and the 17-25 kDa fully cleaved caspase-9.
• Description
  Caspase family of cysteine proteases plays a key role in apoptosis. Caspase-9 is one of the most important caspases among the caspase family members. Upon induction of apoptosis, cytochrome c released from mitochondria associates with procaspase-9/Apaf-1. The complex processes procaspase-9 into a large subunit (35 kDa or 17-25 kDa) and small (10 kDa) by self-cleavage at D315. Activated caspase-9 further cleaves other caspase members including caspase-3, one of the proteases responsible for the proteolytic cleavage of many key proteins in apoptosis. In addition to self-cleavage, procaspase-9 can also be cleaved in vivo by caspase-3 at D330. The process served as a positive feedback to amplify the apoptotic signal in caspase activation pathway.
• Storage Condition
  -20 °C
• Shipping Condition
  gel pack
• Shelf Life
  12 months
• Usage
  For Research Use Only! Not to be used in humans.
• Handling
  The antibody solution should be gently mixed before use.
• Western Blot Verified
  Verified
• Immunocytochemistry Verified
  Not Verified
• Immunofluorescence Verified
  Not Verified
• Immunoprecipitation Verified
  Not Verified
• ELISA Verified
  Not Verified
• ChIP Verified
  Not Verified
• Flow Cytometry Verified
  Not Verified
• Data Sheet Link
• Properties
  If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
• Additional description
  Human and some mouse caspases are active in apoptosis and cell death and even in necrosis and inflammation. CASP Gene and orthologous enzymes have been identifies successfully in the signal transduction cascade and pathways.
• French translation
  anticorps

• Gene target
  Caspase-9  
• Gene symbol
  CARD9, CASP9, CARD19
• Short name
  Caspase-9 Antibody
• Technique
  Antibody, antibodies against human proteins, antibodies for
• Isotype
  Rabbit IgG
• Alternative name
  caspase-9 (antibody to-)
• Alternative technique
  antibodies

Gene info

• Identity
  HGNC:16391
• Gene
  CARD9
• Long gene name
  caspase recruitment domain family member 9
• Synonyms gene name
  • caspase recruitment domain family, member 9
• GenBank acession
  • AF311287
• Locus
  9q34.3
• Discovery year
  2001-08-13
• Entrez gene record
  64170
• Pubmed identfication
  • 11053425
• RefSeq identity
  • NM_052813
• Classification
  • Caspase recruitment domain containing
• VEGA ID
  OTTHUMG00000020925
• Locus Specific Databases
  • LRG_178

Gene info

• Identity
  HGNC:1511
• Gene
  CASP9
• Long gene name
  caspase 9
• Synonyms gene name
  • caspase 9, apoptosis-related cysteine protease
  • caspase 9, apoptosis-related cysteine peptidase
• Synonyms
  • MCH6
  • ICE-LAP6
  • APAF-3
  • PPP1R56
• Synonyms name
  • protein phosphatase 1, regulatory subunit 56
• GenBank acession
  • U60521
• Locus
  1p36.21
• Discovery year
  1996-11-11
• Entrez gene record
  842
• Pubmed identfication
  • 8663294
  • 9390557
• RefSeq identity
  • NM_032996
• Classification
  • Caspases
  • Apoptosome
  • Caspase recruitment domain containing
  • Protein phosphatase 1 regulatory subunits
• VEGA ID
  OTTHUMG00000002256

Gene info

• Identity
  HGNC:28148
• Gene
  CARD19
• Long gene name
  caspase recruitment domain family member 19
• Synonyms gene
  • C9orf89
• Synonyms gene name
  • chromosome 9 open reading frame 89
  • caspase recruitment domain family, member 19
• Synonyms
  • MGC11115
  • bA370F5.1
  • BinCARD
• Synonyms name
  • Bcl10-interacting protein with CARD
• GenBank acession
  • AK057716
• Locus
  9q22.31
• Discovery year
  2004-02-19
• Entrez gene record
  84270
• Pubmed identfication
  • 15637807
• RefSeq identity
  • NM_032310
• Classification
  • Caspase recruitment domain containing
• VEGA ID
  OTTHUMG00000020243

MeSH Data

• Name
  Models, Chemical
• Concept
  Scope note: Theoretical representations that simulate the behavior or activity of chemical processes or phenomena; includes the use of mathematical equations, computers, and other electronic equipment.
• Tree numbers
  • E05.599.495

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