Alkaline phosphatase, placental, human recombinant

• Catalog number
  P1044-10
• Price
  Please ask
• Size
  10 ug

• Synonyms
  ALPP, Alkaline phosphatase Regan isozyme, Placental alkaline phosphatase 1, PLAP-1
• Alternative_names
  ALPP, Alkaline phosphatase Regan isozyme, Placental alkaline phosphatase 1, PLAP-1
• Description
  A hydrolase enzyme responsible for removing phosphate groups
• Recombinant
  Yes
• Source
  Baculovirus
• Purity by SDS PAGE
  > 95%
• Assay
  SDS-PAGE
• Endotoxin Level
  < 1.0 EU per/ug
• Activity Specifications test method
  Measured by its ablity to hydrolyze 1.0 nmole of p-nitrophenyl phosphate (pNPP) per minute at pH 7.5 at 37C.
• Biological activity
  Specific activity is > 2,500 units/mg, and is defined as the amount of enzyme that hydrolyzes 1.0 nmole of p-nitrophenyl phosphate (pNPP) per minute at pH 7.5 at 37C.
• Results
  > 2,500 units/mg
• Unit Definition
  Unit activity is defined as the amount of enzyme that hydrolyzes 1.0 nmole of p-nitrophenyl phosphate (pNPP) per minute at pH 7.5 at 37C.
• Molecular Weight
  53.9 kDa
• Storage Temp
  -20°C
• Shipping
  Gel Pack
• Shelf Life
  12 months
• Appearance
  Liquid
• Physical form description
  In Phosphate Buffered Saline (pH 7.4) containing 10% glycerol
• Background Information
  ALPP, also known as alkaline phosphatase, placental, is a family of dimeric metalloenzymes catalyzing the hydrolysis of monoesters of phosphoric acid. Also, this protein catalyzes a transphosphorylation reaction in the presence of large concentrations of phosphate acceptors. It occurs widely in nature, and are found in many organisms from Escherichia coli to man. Most alkaline phosphatases are homodimeric enzymes and each catalytic site contains three metal ions (two Zn and one Mg), that are necessary for enzymatic activity. Recombinant human ALPP, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.
• Amino acid sequence
  ADLMIIPVEE ENPDFWNREA AEALGAAKKL QPAQTAAKNL IIFLGDGMGV STVTAARILK GQKKDKLGPE LPLAMDRFPY VALSKTYNVD KHVPDSGATA TAYLCGVKGN FQTIGLSAAA RFNQCNTTRG NEVISVMNRA KKAGKSVGVV TTTRVQHASP AGTYAHTVNR NWYSDADVPA SARQEGCQDI ATQLISNMDI DVILGGGRKY MFRMGTPDPE YPDDYSQGGT RLDGKNLVQE WLAKRQGARY VWNRTELMQA SLDPSVTHLM GLFEPGDMKY EIHRDSTLDP SLMEMTEAAL RLLSRNPRGF FLFVEGGRID HGHHESRAYR ALTETIMFDD AIERAGQLTS EEDTLSLVTA DHSHVFSFGG YPLRGSSIFG LAPGKARDRK AYTVLLYGNG PGYVLKDGAR PDVTESESGS PEYRQQSAVP LDEETHAGED VAVFARGPQA HLVHGVQEQT FIAHVMAFAA CLEPYTACDL APPAGTTDHH HHHH
• Handling
  Centrifuge the vial prior to opening.
• Usage
  For Research Use Only! Not to be used in humans
• Datasheet
• Properties
  Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
• Additional source
  Recombinants or rec. proteins
• Group
  recombinants

• Gene target
  Alkaline   phosphatase   placental  
• Gene symbol
  ALPP, ALPG
• Short name
  Alkaline phosphatase, placental, recombinant
• Technique
  Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
• Label
  Alkaline Phosphatase
• Species
  Human, Humans
• Alternative name
  Alkaline phosphatase, placental, H. sapiens Rec.
• Alternative technique
  rec

Gene info

• Identity
  HGNC:439
• Gene
  ALPP
• Long gene name
  alkaline phosphatase, placental
• Synonyms gene name
  • alkaline phosphatase, placental (Regan isozyme)
• Synonyms
  • PALP
  • PLAP
• Synonyms name
  • Regan isozyme
  • placental alkaline phosphatase
• GenBank acession
  • M14169
• Locus
  2q37.1
• Discovery year
  1986-01-01
• Entrez gene record
  250
• Pubmed identfication
  • 3001717
  • 3461452
• RefSeq identity
  • NM_001632
• Classification
  • Alkaline phosphatases
• VEGA ID
  OTTHUMG00000133255

Gene info

• Identity
  HGNC:441
• Gene
  ALPG
• Long gene name
  alkaline phosphatase, germ cell
• Synonyms gene
  • ALPPL2
• Synonyms gene name
  • alkaline phosphatase, placental like 2
• Synonyms
  • GCAP
• Synonyms name
  • germ cell alkaline phosphatase
• GenBank acession
  • J04948
• Locus
  2q37.1
• Discovery year
  1990-07-06
• Entrez gene record
  251
• RefSeq identity
  • NM_031313
• Classification
  • Alkaline phosphatases
• VEGA ID
  OTTHUMG00000133257

MeSH Data

• Name
  Primary Cell Culture
• Concept
  Scope note: The initial culturing of cells derived directly from fresh TISSUES.
• Tree numbers
  • E01.370.225.500.223.500
  • E05.200.500.265.500
  • E05.242.223.500
  • E05.481.500.249.500
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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