Caspase8, His-tag Recombinant

• Background:

  Caspase-8 is a member of the caspase (cysteine aspartate protease) family of proteins, and has been shown to be an executioner protein of apoptosis. Caspase-8 has been associated with chronic inflammation and neurodegenerative disorders including Alzheimer’s, Huntington’s, and Parkinson’s.

• Description:

  Human Caspase 8 (CASP8), GenBank Accession No. NM_001228), a.a. 200 - 496 (end), with C-terminal His-tag, MW=34.5 kDa, expressed in anE. colicell expression system. Procaspase-8 is autocleaved between the prodomain and large protease subunit (p18) . The cleavage product, p30, contains both the large (p18) and small (p10 [His-tag]) protease subunits. p30 is further processed to the p18 and p10 subunits, which dimerize to form active enzyme.

• UniProt:

  Q14790

• Tag:

  C-terminal His-tag

• Purity:

  ≥ 90%

• Format:

  Aqueous buffer solution

• Shipping Conditions:

  -80°C

• Storage Conditions:

  > 6 months at -80°C.

• Calculated Molecular Weight:

  See Description

• Formulation:

  40 mM Tris-HCl, pH 8.0,110 mM NaCl, 2.2 mM KCl, 200 mM imidazole, 3 mM DTT and 20% glycerol.