Immunoglobulin G (IgG) Polyclonal Antibody (Goat), FITC
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Catalog number
PAA544Cp01-1ml-FITC
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Price
Please ask
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Size
1ml
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Description
A Rabbit polyclonal antibody against Goat Immunoglobulin G (IgG). This antibody is labeled with FITC.
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Specifications
Host: Rabbit; Species Reactivity: Goat; Clonality: polyclonal; Tested applications: WB; Concentration: 500ug/ml; Isotype: IgG; Conjugation: FITC
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Additional_information
Sequence of the immunogen: Native Protein IgG; Buffer composition: PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
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Storage_and_shipping
Upon receipt, store at -20°C or -80°C. Prepare working aliqotes prior to storage to avoid repeated freeze-thaw cycles.
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Notes
Research Use Only.
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Properties
If you buy Antibodies supplied by Cloud Clone Corp they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. This Cloud Clone Corp Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. Cloud Clone Corp FITC is highly sensitive to pH extremes.
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Conjugation
Anti-FITC Antibody
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Latin name
Capra aegagrus hircus
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About
Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures. Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.
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Group
Polyclonals and antibodies
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French translation
anticorps
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Gene target
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Gene symbol
IGHV3-71, SEMA4D, FCGR2A
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Short name
Immunoglobulin G (IgG) Polyclonal Antibody (Goat), FITC
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Technique
Polyclonal, Antibody, FITC, Goat, IgG, antibodies against human proteins, antibodies for, Fluorescein, goats, IgGs, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
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Host
Goat, The goat polyclonal antibodies are directed against human genes or rabbit or mouse IgGs (H +L) chain primary antibodies and conjugated for detection. It are very good secondary antibodies that are affinity purified and have a good affinity themselves too. Often KLH is coupled to the antigen so anti KLH can be present in the vial.
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Isotype
IgG, IgG
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Label
FITC
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Species
Goats
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Alternative name
Immunoglobulin G (Immunoglobulin G) polyclonal (antibody to-) (caprine), fluorecein
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Alternative technique
polyclonals, antibodies, caprine, fluorescine, immunoglobulins
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Gene info
Gene info
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Identity
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Gene
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Long gene name
semaphorin 4D
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Synonyms gene
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Synonyms gene name
- chromosome 9 open reading frame 164
- sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4D
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year
1999-06-25
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Immunoglobulin like domain containing
- Semaphorins
- CD molecules
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VEGA ID
Gene info
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Identity
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Gene
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Long gene name
Fc gamma receptor IIa
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Synonyms gene
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Synonyms gene name
- Fc fragment of IgG, low affinity IIa, receptor for (CD32)
- Fc fragment of IgG, low affinity IIa, receptor (CD32)
- Fc fragment of IgG receptor IIa
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year
1988-11-30
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Immunoglobulin like domain containing
- CD molecules
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VEGA ID
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Locus Specific Databases
MeSH Data
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Name
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Concept
Scope note:
A method for the study of certain organic compounds within cells, in situ, by measuring the light intensities of the selectively stained areas of cytoplasm. The compounds studied and their locations in the cells are made to fluoresce and are observed under a microscope.
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Tree numbers
- E01.370.225.500.386
- E05.196.712.516.600.240
- E05.200.500.386
- E05.242.386
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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