MMP-1 Recombinant Protein

• Catalog number
  40-143
• Price
  Please ask
• Size
  0.002 mg, 0.01 mg

• About recombinant protein
  Matrix metalloproteinases (MMPs) are a family of endoproteases that require zinc and calcium for expressing catalytic activity. These enzymes play a central role in the maintenance and remodeling of the extracellular matrix. Elevated expression of their activity, caused either by up-regulation of their expression or down-regulation of their cognate inhibitors, has been implicated in various degenerative disorders, including arthritis, cardiovascular disease, skeletal growth-plate disorders, and cancer metastasis. MMP-1 is a secreted collagenase with specificity toward Type I, II, III, VII, and X collagens. Recombinant human MMP-1 is a 42.7 kDa protein containing the entire catalytic N-terminal domain and the C-terminal domain which is involved in substrate specificity and in binding TIMP-1.
• Other name of the recombinant
  CLG, CLGN, CLG, Interstitial collagenase, Fibroblast collagenase, MMP-1
• Recombinant in
  E. coli
• SEQUENCE
  MFVLTEGNPR WEQTHLTYRI ENYTPDLPRA DVDHAIEKAF QLWSNVTPLT FTKVSEGQAD IMISFVRGDH RDNSPFDGPG GNLAHAFQPG PGIGGDAHFD EDERWTNNFR EYNLHRVAAH ELGHSLGLSH STDIGALMYP SYTFSGDVQL AQDDIDGIQA IYGRSQNPVQ PIGPQTPKAC DSKLTFDAIT TIRGEVMFFK DRFYMRTNPF YPEVELNFIS VFWPQLPNGL EAAYEFADRD EVRFFKGNKY WAVQGQNVLH GYPKDIYSSF GFPRTVKHID AALSEENTGK TYFFVANKYW RYDEYKRSMD PGYPKMIAHD FPGIGHKVDA VFMKDGFFYF FHGTRQYKFD PKTKRILTLQ KANSWFNCRK N
• Activity
  MMP - 1 activity was measured by its ability to cleave a chromogenic peptide MMP - 1 substrate at room temperature. At an MMP - 1 concentration of 2.5 μg/mL, 50% cleavage was achieved at an incubation time of approximately 25 minutes
• Keep at
  The lyophilized MMP-1 recombinant protein is stable for at least 2 years from date of receipt at -20˚C. Reconstituted MMP-1 is stable for at least 3 months when stored in working aliquots with a carrier protein at -20˚C. As with any protein, exposing MMP-1 recombinant protein to repeated freeze / thaw cycles is not recommended. When working with proteins care should be taken to keep recombinant protein at a cool and stable temperature.
• Rec PROTEIN GI
  225543094
• Accession nr
  NP_001139410.1
• Pureity
  Greater than 90% by SDS-PAGE gel and HPLC analyses.Endotoxin level is less than 0.1 ng per μg (1EU/μg).
• About
  This product is for research use only.
• Protein s origin
  Human
• Vial
  Lyophilized
• NCBI ID
  4312
• Gene name
  matrix metallopeptidase 1 (interstitial collagenase)
• Source
  Homo sapiens
• Uniprot
  P03956
• Pictures of recombinant
• Rec pdf
• Additional source
  Recombinants or rec. proteins
• Group
  recombinants

• Gene target
  MMP-1   Protein  
• Gene symbol
  MMP1
• Short name
  MMP-1 Recombinant Protein
• Technique
  Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. proscience advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
• Alternative name
  MMP-1 Rec. Protein
• Alternative technique
  rec

Gene info

• Identity
  HGNC:7155
• Gene
  MMP1
• Long gene name
  matrix metallopeptidase 1
• Synonyms gene
  • CLG
• Synonyms gene name
  • matrix metalloproteinase 1 (interstitial collagenase)
• Synonyms name
  • interstitial collagenase
• GenBank acession
  • X54925
• Locus
  11q22.2
• Discovery year
  1986-01-01
• Entrez gene record
  4312
• RefSeq identity
  • NM_002421
• Classification
  • M10 matrix metallopeptidases
  • Receptor ligands
• VEGA ID
  OTTHUMG00000048192

MeSH Data

• Name
  Primary Cell Culture
• Concept
  Scope note: The initial culturing of cells derived directly from fresh TISSUES.
• Tree numbers
  • E01.370.225.500.223.500
  • E05.200.500.265.500
  • E05.242.223.500
  • E05.481.500.249.500
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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