Goat Anti-Rabbit IgG(H+L) Alexa Fluor 488-conjugated
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Catalog number
S0018
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Price
Please ask
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Size
100µL
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Type
secondary antibody
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Shipping
Ice bag
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Antigen
Goat Anti-Rabbit IgG(H+L) Alexa Fluor 488-conjugated
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Reactivity
Rabbit
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Clonality
Polyclonal
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Application
WB,IF/ICC,FCM
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Application_advice
IF/ICC 1:100-1:500
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Conjugate
Alexa Fluor 488
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Immunogen
The antiserum was developed in goat using the Rabbit IgG as the immunogen.
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Format
Liquid
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Concentration
1mg/ml
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Fragment
Fab fragment
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Storage_Buffer
0.01M Sodium phosphate, 0.25M NaCl, 50% glycerol, pH7.6. Store at -20℃,avoid repeated thawing and freezing.
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Properties
For facs or microscopy Alexa 1 conjugate. Alexa Fluor 488 has the same range to that of fluorescein isothiocyanate (FITC), yet the Goat Anti-Rabbit IgG(H+L) -conjugated has a very high photo stability. As a result of this photo stability, it has turned into an antibody for fluorescent microscopy and FACS FLOW cytometry. It is distinguished in the FL1 of a FACS-Calibur or FACScan. Also Alexa Fluor 488 is pH stable.
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Conjugation
Alexa Fluor
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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Latin name
Capra aegagrus hircus, Oryctolagus cuniculus
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About
Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures. Rabbits are used for polyclonal antibody production by Affinity Biosciences. Rabbit antibodies are very stable and can be stored for several days at room temperature. Affinity Biosciences adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
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Gene target
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Short name
Goat Anti-Rabbit IgG(H+L) -conjugated
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Technique
Rabbit, anti-, anti, Goat, IgG, antibody to, antibodies, antibody Conjugates, goats, IgGs
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Host
Goat, The goat polyclonal antibodies are directed against human genes or rabbit or mouse IgGs (H +L) chain primary antibodies and conjugated for detection. It are very good secondary antibodies that are affinity purified and have a good affinity themselves too. Often KLH is coupled to the antigen so anti KLH can be present in the vial. Rabbits
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Isotype
IgG1, IgG
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Label
ALEXA FLUOR 488
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Species
Goats
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Alternative name
caprine Antibody tohost: rabbit Immunoglobulin G(heavy + light chain) Alexa Fluor 488-coupled
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Alternative technique
rabbit-anti, antibodies, caprine, immunoglobulins
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MeSH Data
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Name
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Concept
Scope note:
Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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