Rat Anti-Mouse CD8a/Ly-2-FITC

• Catalog number
  GEN673185
• Price
  Please ask
• Size
  500ug

• Also known as
  CD8 alpha /Lyt-2
• Other names
  N/A
• Category
  Secondary Antibodies
• Subcategory
  Mnoclonal antibodies
• Gene name
  N/A
• Gene name synonims
  N/A
• Other gene names
  N/A
• Clonality
  Monoclonal
• Immunoglobulin isotype
  Rat (LOU/Ws1/M) IgG2a kappa
• Clone
  53-6.7
• Host organism
  Rat (Rattus norvegicus)
• Species reactivity
  Mouse (Mus musculus); Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby.
• Specificity and cross reactivity
  alpha chain of the murine CD8 heterodimer, Mr 32-34 kDa In the mouse, CD8 exists in two forms: (i) a CD8 heterodimer composed of an alpha chain (CD8 alpha /Lyt-2) and a beta chain (CD8 beta /Lyt-3); and (ii) a homodimer of two alpha chains. The heterodimer is found on the surface of essentially all thymocytes and the "suppressor/cytotoxic" subpopulation of mature T lymphocytes. Subsets of intestinal intraepithelial lymphocytes express CD8 alpha without CD8 beta. It has been suggested that CD8^+ beta ^- T cells mature extrathymically, while development of the CD8 alpha ^+ beta ^+ population of T cells is thymus-dependent. CD8 acts as a coreceptor with MHC Class I-restricted T cell receptors in antigen recognition and positive selection of MHC class I-restricted CD8^+ T cells. In vivo and in vitro treatment with the 53-6.7 monoclonal antibody effectively depletes CD8 alpha ^+ cells. The 53-6.7 monoclonal antibody also blocks allogeneic help specific for class I MHC antigens and T cell responses to IL-2. 1-8; Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited. This particular antibody might cross react with speacies outside of the listed ones.
• Purification method
  N/A
• Form Appearance
  Fluorescein (FITC) Conjugate
• Concentration
  N/A
• Storage and shipping
  The purified (UNLB) antibody is supplied as 0.5 mg of purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added. Store at 2- 8 degree C. The fluorescein (FITC) conjugate is supplied as 0.5 mg or 0.1 mg in 1.0 mL of PBS/NaN3. Store at 2- 8 degree C. The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store the antibody ats should be kept in the range of 1-7 degrees Celsius.. The R-phycoerythrin (R-PE) conjugate is supplied as 0.1 mg in 1.0 mL or 0.2 mg in 2.0 mL of PBS/NaN3 and a stabilizing agent. Store the antibody ats should be kept in the range of 1-7 degrees Celsius.. Do not freeze! The allophycocyanin (APC) conjugate is supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store the antibody ats should be kept in the range of 1-7 degrees Celsius.. Do not freeze! The Spectral Red (SPRD), R-phycoerythrin-Cyanine 7 (R-PE-CY 7), R-phycoerythrin-Cyanine 5.5 (R-PE-CY 5.5), allophycocyanin-Cyanine 5.5 conjugate (APC-CY 5.5), allophycocyanin-Cyanine 7 (APC-CY 7) and R-phycoerythrin-Texas Red (R-PE-TXRD) conjugates are supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store the antibody ats should be kept in the range of 1-7 degrees Celsius.. Do not freeze! The low endotoxin, azide-free (LE/AF) antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mL of PBS. Aliquot and store at or below Store the antibody at -20 degrees Celsius.. The Cyanine 5 (CY 5) conjugate is supplied as 0.1 mg in 1.0 mL of PBS/NaN3. Store the antibody ats should be kept in the range of 1-7 degrees Celsius.. Protect conjugated forms from light. Each reagent is stable for the period shown on the bottle label if stored as directed.
• Tested applications
  Flow Cytometry; Immunohistochemistry (Acetone Fixed/Frozen Sections); Immunoprecipitation
• Description
  This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
• Properties
  This MBS Monoclonals Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. MBS Monoclonals FITC is highly sensitive to pH extremes.
• Conjugation
  Anti-FITC Antibody
• Test
  Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
• Latin name
  Mus musculus, Rattus norvegicus
• About
  Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.

• Gene target
  CD8a/Ly-2  
• Short name
  Anti-Mouse CD8a/Ly-2-FITC
• Technique
  anti-, Mouse, anti, FITC, antibody to, antibodies, Fluorescein, mouses
• Host
  Rat
• Label
  FITC
• Species
  Mouse, Mouses, Rats
• Alternative name
  Anti-Rat Anti-Mouse CD8a/Ly-2-FITC
• Alternative technique
  murine, antibodies, fluorescine
• Alternative to gene target
  CD8a molecule, CD8 and Leu2 and MAL and p32, CD8A and IDBG-59987 and ENSG00000153563 and 925, protein homodimerization activity, Extracellular, Cd8a and IDBG-155582 and ENSMUSG00000053977 and 12525, CD8A and IDBG-634911 and ENSBTAG00000021141 and 281060

Product images

Similar products