pcDNA3.0-Flag-CD40LG Plasmid
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Catalog number
PVTB00477-2a
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Price
380.85 USD
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Size
2 ug
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Properties
An anti-flag tag (FLAG fusion protein) is use to detect a FLAG-tag, or FLAG octapeptide, or FLAG epitope that is a polypeptide protein tag that can be added to a protein using recombinant DNA. This FLAG-tags have the sequence DYKDDDDK motiv. These tags are very useful to do protein purification by affinity chromatography. Also separation of recombinant, overexpressed proteins from cell lysates is done by FLAG go HIS tags. FLAGS are also used in the isolation of protein complexes with multiple subunits, because its mild purification procedure tends not to disrupt such complexes. It has been used to enrich proteins of height purity and quality to see the 3D crystal structure with x-ray. Suitable for in vivo use in cells. For electrophorese protein detection rabbit polyclonals anti Flag conjugation are the most suited antibodies.
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Conjugation
Flag
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Kit
Plasmid mini made and maxi DNA purification kits can be silica gel or anion exchange, endotoxin free and are used to produce pure plasmids that are small DNA molecules within a cell separated from chromosomal DNA and can replicate independently. They are most commonly found in bacteria as small circular, double-stranded DNA molecules; however, plasmids are sometimes present in archaea and eukaryotic organisms. In nature, plasmids often carry genes that may benefit the survival of the organism, for example antibiotic resistance. While the chromosomes are big and contain all the essential information for living, plasmids usually are very small and contain only additional information. Artificial plasmids are widely used as vectors in molecular cloning, serving to drive the replication of recombinant DNA sequences within host organisms.
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Gene target
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Gene symbol
CD40LG
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Short name
pcDNA3 - -CD40LG Plasmid
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Technique
plasmid, plasmids in 1
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Label
Flag
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Alternative name
pcDNA3.0-Flag-CD40 molecule, tumor necrosis factor receptor superfamily member 5 ligand Plasmid
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Alternative technique
plasmids
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Alternative to gene target
CD40 ligand, CD154 and CD40L and gp39 and hCD40L and HIGM1 and IGM and IMD3 and T-BAM and TNFSF5 and TRAP, CD40LG and IDBG-87653 and ENSG00000102245 and 959, protein binding, Extracellular, Cd40lg and IDBG-147440 and ENSMUSG00000031132 and 21947, CD40LG and IDBG-631246 and ENSBTAG00000017843 and 282387
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Gene info
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Identity
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Gene
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Long gene name
CD40 ligand
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Synonyms gene
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Synonyms gene name
- tumor necrosis factor (ligand) superfamily, member 5 (hyper-IgM syndrome)
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year
1989-06-30
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- CD molecules
- Tumor necrosis factor superfamily
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VEGA ID
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Locus Specific Databases
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