Recombinant Human Nuclear Transcription Factor Y Subunit a/NFYA (N-GST)

• Catalog number
  CE78-1000
• Price
  Please ask
• Size
  1 mg

• Description
  Recombinant Human Nuclear TF Y subunit alpha is produced by our E.coli expression system and the target gene encoding Met1-Ser318 is expressed with a GST tag at the N-terminus.
• Species reactivity
  Human
• Origin
  Escherichia coli
• Peptide sequence
  MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPKSDLVPRGSPEFMEQYTANSNSSTEQIVVQAGQIQQQVQGQPLMVQVSGGQLITSTGQPIMVQAVPGGQGQTIMQVPVSGTQGLQQIQLVPPGQIQIQGGQAVQVQGQQGQTQQIIIQQPQTAVTAGQTQTQQQIAVQGQQVAQTAEGQTIVYQPVNADGTILQQVTVPVSGMITIPAASLAGAQIVQTGANTNTTSSGQGTVTVTLPVAGNVVNSGGMVMMVPGAGSVPAIQRIPLPGAEMLEEEPLYVNAKQYNRILKRRQARAKLEAEGKIPKERRKYLHESRHRHAMARKRGEGGRFFSPKEKDSPHMQDPNQADEEAMTQIIRVS
• Estimated molecular weight
  60,58 kDa
• Protein purity
  Greater than 95% as determined by reducing SDS-PAGE.
• Endotoxin level
  Less than 0.1 ng/µg (1 IEU/µg) as determined by LAL test.
• Shipping condition
  Ambient/Room Temperature
• Package form
  Lyophilized from a 0.2 µm filtered solution of 20mM PB, 150mM NaCl, pH 7.2.
• Storage conditions
  Lyophilized protein should be stored at below -20°C, though stable at room temperature for 3 weeks.Reconstituted protein solution can be stored at 4-7°C for 2-7 days.Aliquots of reconstituted samples are stable at below -20°C for 3 months.
• Reconstitution conditions
  Always centrifuge tubes before opening. Do not mix by vortex or pipetting.It is not recommended to reconstitute to a concentration less than 100 µg/ml.Dissolve the lyophilized protein in ddH2O.Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
• UniProt number
  P23511
• Other related products
• Additional description
  Aplha, transcription related growth factors and stimulating factors or repressing nuclear factors are complex subunits of proteins involved in cell differentiation. Complex subunit associated factors are involved in hybridoma growth, Eosinohils, eritroid proliferation and derived from promotor binding stimulating subunits on the DNA binding complex. NFKB 105 subunit for example is a polypetide gene enhancer of genes in B cells. The activation of transcription factor subunits is the first step of gene expression, in which a particular segment of DNA is copied into RNA (mRNA) by the enzyme RNA polymerases. Transcription factors, unites and elongations can be RNA and DNA nucleic acids, base pairs of nucleotides . Converting from DNA to RNA is made by enzymatic reactions. During transcription, a DNA sequence is read by an RNA polymerase, which produces a complementary, anti-parallel RNA strand called a primary transcript. Transcriptions are key functions in signal transduction pathways. Signaling ligand binding transcription factors play an important role in transduction cascades.
• Properties
  Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
• Source
  Recombinants or rec. proteins
• Group
  recombinants

• Gene target
  Transcription   Factor   Subunit   a/NFYA   N-GST  
• Gene symbol
  MGST3, GH1, POLM, NRAS, NFYA, SDC3, PIGN, WASL, MAP6, HLA-N
• Short name
  Recombinant Transcription Factor Y Subunit a/NFYA (N-GST)
• Technique
  Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. novo advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
• Species
  Human, Humans
• Alternative name
  Human Nuclear TF Y subunit alpha/NFYA(N-GST)
• Alternative technique
  rec

Gene info

• Identity
  HGNC:7064
• Gene
  MGST3
• Long gene name
  microsomal glutathione S-transferase 3
• Synonyms
  • GST-III
• Synonyms name
  • microsomal glutathione S-transferase III
  • microsomal GST-3
  • microsomal GST-III
• GenBank acession
  • AF026977
• Locus
  1q24.1
• Discovery year
  1997-10-16
• Entrez gene record
  4259
• Pubmed identfication
  • 9278457
• RefSeq identity
  • NM_004528
• Classification
  • Microsomal glutathione S-transferases
• VEGA ID
  OTTHUMG00000034627

Gene info

• Identity
  HGNC:4261
• Gene
  GH1
• Long gene name
  growth hormone 1
• Synonyms
  • GH-N
  • GHN
  • GH
  • hGH-N
• Synonyms name
  • pituitary growth hormone
  • somatotropin
• GenBank acession
  • M13438
• Locus
  17q23.3
• Discovery year
  2001-06-22
• Entrez gene record
  2688
• Pubmed identfication
  • 6306568
• RefSeq identity
  • NM_000515
• Classification
  • Growth hormone family
• VEGA ID
  OTTHUMG00000172293

Gene info

• Identity
  HGNC:9185
• Gene
  POLM
• Long gene name
  DNA polymerase mu
• Synonyms gene name
  • polymerase (DNA directed), mu
  • polymerase (DNA) mu
• Synonyms
  • Tdt-N
• Synonyms name
  • Pol iota
• GenBank acession
  • AF176097
• Locus
  7p13
• Discovery year
  2000-02-16
• Entrez gene record
  27434
• Pubmed identfication
  • 10982892
• RefSeq identity
  • NM_013284
• Classification
  • DNA polymerases
  • MicroRNA protein coding host genes
• VEGA ID
  OTTHUMG00000155353

Gene info

• Identity
  HGNC:7989
• Gene
  NRAS
• Long gene name
  NRAS proto-oncogene, GTPase
• Synonyms gene name
  • neuroblastoma RAS viral (v-ras) oncogene homolog
  • neuroblastoma RAS viral oncogene homolog
• Synonyms
  • N-ras
• GenBank acession
  • BC005219
• Locus
  1p13.2
• Discovery year
  2001-06-22
• Entrez gene record
  4893
• Pubmed identfication
  • 6705568
  • 11524732
• RefSeq identity
  • NM_002524
• Classification
  • RAS type GTPase family
• VEGA ID
  OTTHUMG00000012059
• Locus Specific Databases
  • NRASbase: Mutation registry for autoimmune lymphoproliferative syndrome type IV
  • LRG_92

Gene info

• Identity
  HGNC:7804
• Gene
  NFYA
• Long gene name
  nuclear transcription factor Y subunit alpha
• Synonyms gene name
  • nuclear transcription factor Y, alpha
• Synonyms
  • HAP2
  • CBF-B
  • NF-YA
• Locus
  6p21.1
• Discovery year
  1991-11-08
• Entrez gene record
  4800
• Pubmed identfication
  • 1774067
  • 9612081
• VEGA ID
  OTTHUMG00000014669

Gene info

• Identity
  HGNC:10660
• Gene
  SDC3
• Long gene name
  syndecan 3
• Synonyms gene name
  • syndecan 3 (N-syndecan)
• Synonyms
  • N-syndecan
  • SYND3
• Synonyms name
  • syndecan proteoglycan 3
• GenBank acession
  • AF248634
• Locus
  1p35.2
• Discovery year
  1994-05-16
• Entrez gene record
  9672
• Pubmed identfication
  • 1556152
  • 11527150
• RefSeq identity
  • NM_014654
• Classification
  • Syndecans
• VEGA ID
  OTTHUMG00000043646

Gene info

• Identity
  HGNC:8967
• Gene
  PIGN
• Long gene name
  phosphatidylinositol glycan anchor biosynthesis class N
• Synonyms gene name
  • phosphatidylinositol glycan, class N
• Synonyms
  • MCD4
  • PIG-N
• GenBank acession
  • AF109219
• Locus
  18q21.33
• Discovery year
  2000-05-11
• Entrez gene record
  23556
• Pubmed identfication
  • 10069808
  • 10574991
• RefSeq identity
  • NM_176787
• Classification
  • Phosphatidylinositol glycan anchor biosynthesis
• VEGA ID
  OTTHUMG00000180098

Gene info

• Identity
  HGNC:12735
• Gene
  WASL
• Long gene name
  WASP like actin nucleation promoting factor
• Synonyms gene name
  • Wiskott-Aldrich syndrome-like
  • Wiskott-Aldrich syndrome like
• Synonyms
  • N-WASP
  • NWASP
  • WASPB
• GenBank acession
  • D88460
• Locus
  7q31.32
• Discovery year
  1998-10-14
• Entrez gene record
  8976
• Pubmed identfication
  • 9422512
  • 9322739
  • 23527602
• RefSeq identity
  • NM_003941
• Classification
  • Wiskott-Aldrich Syndrome protein family
• VEGA ID
  OTTHUMG00000157346

Gene info

• Identity
  HGNC:6868
• Gene
  MAP6
• Long gene name
  microtubule associated protein 6
• Synonyms
  • KIAA1878
  • STOP
  • FLJ41346
  • MAP6-N
• GenBank acession
  • AK123340
• Locus
  11q13.5
• Discovery year
  1998-02-26
• Entrez gene record
  4135
• Pubmed identfication
  • 10516426
  • 12231625
• RefSeq identity
  • NM_033063
• VEGA ID
  OTTHUMG00000165343

Gene info

• Identity
  HGNC:19406
• Gene
  HLA-N
• Long gene name
  major histocompatibility complex, class I, N (pseudogene)
• Synonyms
  • HLA-30
• GenBank acession
  • M96329
• Locus
  6p22.1
• Discovery year
  2002-10-23
• Entrez gene record
  267014
• Pubmed identfication
  • 10452889
  • 1517563
• RefSeq identity
  • NG_009940
• Classification
  • Histocompatibility complex
• VEGA ID
  OTTHUMG00000031167
• Locus Specific Databases
  • IMGT/HLA Database

MeSH Data

• Name
  Primary Cell Culture
• Concept
  Scope note: The initial culturing of cells derived directly from fresh TISSUES.
• Tree numbers
  • E01.370.225.500.223.500
  • E05.200.500.265.500
  • E05.242.223.500
  • E05.481.500.249.500
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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