Available ordering format Lyophilized
Recommended dilutions for use 1: 10 000 with ECL (WB)
Raised in Rabbit
Storage condition store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Molecular weight (expected | аpparent) 55.76 kDa
Immunogen His-tagged full length recombinant UAGPase of Arabidopsis thaliana, overexpressed and purified from E.coli, UniProt:O64765,, TAIR:AT2G35020. Sequence used for immunization is conserved in both isoforms: UDP-N-acetylglucosamine diphosphorylase 1 (Q940S3) and (O64765)
Protein number O64765
Possible reactivity Glycine soja, Medicago truncatula, Morus notabilis, Populus trichocarpa, Theobroma cacao, Zea mays, for more species, please inquire
Connected products collection of antibodies to carbohydrate metabolismrecommended secondary antibodyPlant protein extraction buffer Secondary antibodies
Scientific context UAGPase (UDP-GlcNAc pyrophosphorylase) is involved in the biosynthesis of UDP-glucosamine, an essential precursor for glycoprotein and glycolipid synthesis and is also used for regulatory protein modification in signaling pathways. The enzyme is localized in a cytoplasm. Alternative names: N-acetylglucosamine-1-phosphate uridylyltransferase 2, UDP-N-acetylgalactosamine diphosphorylase 2, UTP--glucose-1-phosphate uridylyltransferase 2, AGX.
TAIR number AT2G35020
No reactivity no confirmed exceptions from predicted reactivity known in the moment
Verified reactivity Arabidopsis thaliana, Hordeum vulgare, Nicotiana tabacum
References Kleczkowski LA & Decker DD (2015) Sugar activation for production of nucleotide sugars as substrates for glycosyltransferases in plants. J. Appl. Glycosci. (in press).
How to reconstitute For reconstitution add 50 µl of sterile water.
Supplementary information This antibody is recognizing recombinant UAGPase at 0.25 pmol.
Verified applications western blot (WB)