RPMI 1640 w/ L-Glutamine w/ 25 mM Hepes - 500ml
-
Catalog number
LM-R1637/500
-
Price
Please ask
-
Size
40X500ml
-
-
Description
RPMI 1640 w/ L-Glutamine w/ 25 mM Hepes - 500ml is used in cultures in vitro, suspension and monolayer culture of human leukemic cells.
-
-
Shipping conditions
-20°C or dry ice
-
Storage
+ 2 / + 8 ° C
-
Shelflife
12 months
-
State
Liquid
-
Chemical description
Water-based mixture of inorganic and organic compounds
-
Appearance
Physical state at 20 ºC: Liquid ; Appearance: Translucent ; Colour: Orange
-
Density at 20 ºC
1040 kg/m³
-
Relative density at 20 ºC
1.04
-
PH
7.3 ± 0.3
-
Osmolality
294mOsm/kg ±10 %
-
Cell growth test
The media is tested for the ability to support SP2/0-Ag14 cell growth
-
Composition
NA
-
Sterility
Sterilizing filtration
-
Personal precautions
Wear clothes adapted to the manipulation of serum to avoid contamination (e.g. : gloves, mask, hygiene cap, overall…)
-
Uses
Usage biotechnologique. Uniquement pour usage professionnel.
-
-
-
Ordering
To order RPMI 1640 w/ L-Glutamine w/ 25 mM Hepes - 500ml, please use the Cat. Nr.LM-R1637/500 and submit your purchase order by email or by fax. A discount is available for larger or bulk quantities, please contact us for more information
-
Tips
Our specialists recommend you to follow carefully the pre-registered instructions for RPMI 1640 w/ L-Glutamine w/ 25 mM Hepes - 500ml
-
Buffer
25mM HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid ) pH7 solution buffers are a media with a zwitterion organic chemical buffering agent; one of the twenty Good's buffers. HEPES DMEM medium is widely used in cell culture, largely because it is better at maintaining physiological pH 7 tot 7,5 despite changes in carbon dioxide concentration (produced by cellular respiration) when compared to bicarbonate buffers, which are also commonly used in cell culture. The dissociation of water decreases with falling temperature, but the dissociation constants (pK) of many other buffers do not change much with temperature. HEPES, 25 mM in RPMI and DMEM is like water in that its dissociation decreases as the temperature decreases. This makes HEPES RPMI 25 milli-molar media with or without L-glutamine a more effective buffering agent for maintaining enzyme structure and function at low temperatures.
Similar products
