Mouse DEFa5(Defensin Alpha 5, Paneth Cell Specific) ELISA Kit

• Catalog number
  ELK2462
• Price
  Please ask
• Size
  96T

• Alternative Names
  DEF-A5; DEF5; HD-5
• Assay Type
  Sandwich
• Sensitivity
  13.1pg/mL
• Standard
  2000pg/mL
• Detection range
  31.2-2000pg/mL
• Assay length
  3h
• Research Area
  Infection immunity;Hepatology;Gastroenterology;
• Test principle
  The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Defensin Alpha 5, Paneth Cell Specific (DEFα5). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Defensin Alpha 5, Paneth Cell Specific (DEFα5). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Defensin Alpha 5, Paneth Cell Specific (DEFα5), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Defensin Alpha 5, Paneth Cell Specific (DEFα5) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• Description
  The Mouse DEFa5(Defensin Alpha 5, Paneth Specific) ELISA Kit is a α- or alpha protein sometimes glycoprotein present in blood. For cells, cell lines and tissues in culture till half confluency. human, mouse or other beta β- and alpha α- defensins are expressed by neutrophils ( α- ), lymphocytes and epithelial cells. DEFA DEFB genes defend the host against pathogens. GENTAUR supplies anti-defensins, monclonals, polyclonals antisera and recombinant DEFs or peptides of defensin.
• Properties
  E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays, E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays
• Test
  ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED, Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
• Latin name
  Mus musculus

• Gene target
  DEFa5   Defensin   Alpha   Paneth   Specific  
• Gene symbol
  DEFA5
• Short name
  Mouse DEFa5(Defensin Alpha 5, Paneth Specific) ELISA Kit
• Technique
  ELISA kit, ELISA, Mouse, ELISAs Enzyme-linked immunosorbent assay 90320007 SNOMED code are used by the medical researcher for detection of human, mouse, … proteins are supplied in coated 96 well plates to be stored at +4°C. ELISA test kits can be sandwich ELISA. In sandwich ELISAS Enzyme-linked immunosorbent assay 9we use captor and detector antibodies. In single antibody ELISAs the antigen is coated and only a detector antibody is used. Traditional competition antigen ELISAs are coated with a captor antibody and a competitive antigen is labelled with the chromogen. In this case the ratio on your graph will be inverse proportional with the antigen present in your sample. A sign detected by the Colorimetric detection OD optical density absorbance at 450 nm between 0 and 4 with an ELISA reader for 450 nm absortion. Genprice Inc. the recognized authority tax ID 45-4304622 D-U-N-S number - 078440800. MeSH code E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay. PROMT code 3841327 Immunoabsorbent assay tests. ELISA tests are enzyme linked immunoassays to detect human, mouse or other proteins in serum, plasma, urine or biological fluids. MeSH code E05 478 566 350 170 for Enzyme-Linked Immunosorbent Assay or ELISA kits. Also Enzyme-linked immunoassay, competitive have the SNOMED name 61461007 and the PROMT code for ELISA readers is 3841330. This is the desigation of other diagnostic analyzers and tests. mouses
• Host
  mouse, antigen assay ELISA kit 1 Kit (96 Wells)
• Species
  Mouse, Mouses
• Alternative name
  Mouse defensin, alpha 5, Paneth cellular-specific(Defensin a 5, Paneth cellular Specific) Enzyme-linked immunosorbent assay reagent
• Alternative technique
  murine, elisas, kits
• Alternative to gene target
  defensin, alpha 5, Paneth cell-specific, DEF5 and HD-5, DEFA5 and IDBG-6199 and ENSG00000164816 and 1670, Extracellular
• Tissue
  cell

Gene info

• Identity
  HGNC:2764
• Gene
  DEFA5
• Long gene name
  defensin alpha 5
• Synonyms gene
  • DEF5
• Synonyms gene name
  • defensin, alpha 5, Paneth cell-specific
• Synonyms
  • HD-5
• GenBank acession
  • BC069690
• Locus
  8p23.1
• Discovery year
  1993-11-05
• Entrez gene record
  1670
• Pubmed identfication
  • 1429669
• RefSeq identity
  • NM_021010
• Classification
  • Defensins, alpha
• VEGA ID
  OTTHUMG00000090383

MeSH Data

• Name
  Enzyme-Linked Immunosorbent Assay
• Concept
  Scope note: An immunoassay utilizing an antibody labeled with an enzyme marker such as horseradish peroxidase. While either the enzyme or the antibody is bound to an immunosorbent substrate, they both retain their biologic activity; the change in enzyme activity as a result of the enzyme-antibody-antigen reaction is proportional to the concentration of the antigen and can be measured spectrophotometrically or with the naked eye. Many variations of the method have been developed.
• Tree numbers
  • E05.478.566.350.170
  • E05.478.566.380.360
  • E05.478.583.400.170
  • E05.601.470.350.170
  • E05.601.470.380.360
• Qualifiers
  ethics, mortality, psychology, trends, veterinary, history, classification, economics, instrumentation, methods, nursing, standards, adverse effects, statistics & numerical data

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