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Also known as
a-Actin, Skeletal Muscle
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Other names
actin, alpha skeletal muscle; Actin, alpha skeletal muscle; actin, alpha skeletal muscle; a-actin; alpha-actin-1; actin, alpha 1, skeletal muscle; Alpha-actin-1
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Category
Antibodies
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Subcategory
Mnoclonal antibodies
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Gene name
ACTA1; ACTA1; N/A; ACTA
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Gene name synonims
ACTA1; ACTA1; N/A; ACTA
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Other gene names
ACTA1; ACTA1; N/A; ACTA
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Clonality
Monoclonal
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Immunoglobulin isotype
IgM,k
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Clone
0.N.4
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Host organism
Mouse (Mus musculus)
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Source organism
Human (Homo sapiens)
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Species reactivity
Bovine, Frog, Guinea Pig, Human (Homo sapiens), Rabbit (Oryctolagus cuniculus), Rat (Rattus norvegicus), Sheep; Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby.
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Specificity and cross reactivity
Recognizes human alpha-skeletal and alpha-cardiac (sarcomeric) actins as well as neoplasms derived from normal tissues (i.e. rhabdomyosarcomas). Shows no crossreaction with smooth muscle actin. Species Crossreactivity: bovine, rabbit, sheep, rat, guinea pig, and frog. ; Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited. This particular antibody might cross react with speacies outside of the listed ones.
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Purification method
Purified by ammonium sulfate precipitation.
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Form Appearance
Supplied as a liquid in PBS, pH 7.4. Also available with BSA and azide. See A0760-24.
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Concentration
N/A
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Storage and shipping
Store the antibody at +4 degrees Celsius for short term storage.. For long-term storage, For optimal long term storage, the antibody should be kept at -20 degrees Celsius. Aliquots are stable for at least 12 months the antibody should be stored at -20 degrees Celsius.. Prior to use, briefly centrifuge the original vial after thawing and before lid. If further dilution(s) are needed, they can be done with the appropriate assay buffer.
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Tested applications
Immunohistochemistry (IHC), Immunofluorescence (IF)
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Description
Sodium azide NaN3 is often used to prevent antibody degradation in 0,02% W/V concentrations. MBS Monoclonals supplies azide free antibodies on request. Recently MBS Monoclonals made this a-Actin, Skeletal Muscle (BSA & ) available without azide. It will stick less and is more suitable for ELISA or in vivo studies. However you need to store the @VV@ frozen and ship on dry ice.
