Glutaredoxin 1 Antibody (Clone 28C3)
-
Catalog number
6211-100
-
Price
Please ask
-
Size
100 μl
-
-
Antibody Target
Glutaredoxin 1
-
Target Alternative Name
Thioltransferase-1, GLRX, GRX
-
Antibody Type
Monoclonal
-
Clone
Clone 28C3
-
Species Reactivities
Human
-
Immunogen Sequence
Recombinant human protein purified from E.coli
-
Accession
P35754
-
Gene Id
2745
-
Appearance
Colorless liquid
-
Form
Liquid
-
Concentration
1 mg/ml
-
Formulation
100 µl of antibody in HEPES with 0.15 M NaCl, 0.01 % BSA, 0.03 % sodium azide, and 50 % glycerol
-
Purification
Ammonium sulphate precipitation
-
Application
IP, ELISA.
-
Positive Control
WB and IP: Jurkat cell lysate
-
Application And Usages
IP: 1-2 µl, ELISA.
-
Description
Glutaredoxin (Grx), also known as thiol transferase, is a small heat-stable oxidoreductase. Grxs form part of the glutaredoxin system, comprising NADPH, GSH and glutathione reductase, which transfers electrons from NADPH to glutaredoxins via GSH. First recovered in E.coli as GSH-dependent hydrogen donors for ribonucleotide reductase, Grx catalyzes GSH-disulfide oxido-reductase via two redox-active cysteine residues. The active sequence (Cys-Pro-Tyr-Cys) is conserved in a variety of species. The 12 kDa dithiol protein has a role in reduction of mixed disulfides in cells exposed to oxidative stress. Has a glutathione-disulfide oxidoreductase activity in the presence of NADPH and glutathione reductase. Reduces low molecular weight disulfides and proteins.
-
Storage Condition
-20 °C
-
Shipping Condition
gel pack
-
Shelf Life
12 months
-
Usage
For Research Use Only! Not to be used in humans.
-
Handling
The antibody solution should be gently mixed before use.
-
Western Blot Verified
Verified
-
Immunocytochemistry Verified
Not Verified
-
Immunofluorescence Verified
Not Verified
-
Immunoprecipitation Verified
Verified
-
ELISA Verified
Not Verified
-
ChIP Verified
Not Verified
-
Flow Cytometry Verified
Not Verified
-
-
Properties
If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
-
French translation
anticorps
-
Gene target
-
Short name
Glutaredoxin 1 Antibody (Clone 28C3)
-
Technique
Antibody, antibodies against human proteins, antibodies for
-
Isotype
Mouse IgG1
-
Alternative name
Glutaredoxin 1 (antibody to-) (clonality 28C3)
-
Alternative technique
antibodies
-
Clone name
28C3
-
MeSH Data
-
Name
-
Concept
Scope note:
Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
-
Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
-
Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Product images
Similar products