Sulforhodamine B Cell Cytotoxicity Assay Kit (Colorimetric)

• Catalog number
  K943-1000
• Price
  Please ask
• Size
  1000 assays

• Summary
  • Detection method- Absorbance (565 nm) • Sample type- Cell culture (adherent) • Species reactivity- Mammalian • Applications- This assay provides a convenient and non-radioactive alternative to carry out cytotoxicity assays. This assay relies on the ability of SRB to bind cellular protein components and measure the total biomass.
• Detection Method
  Detection method- Absorbance (565 nm)
• Species Reactivity
  Species reactivity- Mammalian
• Applications
  This assay provides a convenient and non-radioactive alternative to carry out cytotoxicity assays. This assay relies on the ability of SRB to bind cellular protein components and measure the total biomass.
• Sample Type
  Cell cultures (adherent)
• Features Benefits
  • Simple procedure; takes less than 2 hours • Fast, sensitive and convenient
• Storage Conditions
  -20°C
• Shipping Conditions
  gel pack
• Shelf life
  12 months
• Background
  Sulforhodamine B (SRB) cell cytotoxicity assay is one of the most widely methods used to detect cell viability or drug cytotoxicity. This assay relies on the ability of SRB to bind cellular protein components and measure the total biomass. SRB is a bright-pink aminoxanthene dye that can form an electrostatic complex with basic amino acid residues of proteins in slightly acidic conditions but it can dissociate under basic conditions. It has been widely used for drug toxicity screening against different types of cancerous and non-cancerous cell lines. In addition, this assay is independent of cell metabolic activity and therefore should show less interference by the testing compounds. Since the binding of SRB is stoichiometric, the incorporated dye released from stained cells after washing is directly proportional to the cell biomass and can be measured at 565 nm. BioVision’s SRB cell cytotoxicity assay kit is simple, accurate, reproducible and sensitive. This kit offers an excellent and efficient method for in vitro cytotoxicity studies as well as high-throughput drug screening that can detect between 5,000-50,000 cells per well
• Data Sheet Link
• Test
  Biovision supplies other types of Assays as 1.
• Additional description
  For cells, cell lines and tissues in culture till half confluency.
• Properties
  Colorimetric assays or detection use UV absorption or enzymatic color reaction.

• Gene target
  Sulforhodamine   Cytotoxicity   Kit  
• Short name
  Sulforhodamine B Cytotoxicity Assay Kit (Colorimetric)
• Technique
  colorimetric, Assay, assays, colorimetry
• Host
  Assay
• Alternative name
  Sulforhodamine B cellular Cytotoxicity test reagent (colorimetry)
• Alternative technique
  assays, arrays, kits
• Alternative to gene target
  v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
• Tissue
  cell

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