Phospho-SMAD4 Antibody (pT277)
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Catalog numberF48451-0.4ML
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Price:
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Size0.4 ml
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CategoryAntibody
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ConcentrationIn 1X PBS, pH 7.4, with 0.09% sodium azide
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FormAntigen affinity purified
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ConjugationUnconjugated
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ClonePolyclonal antibody
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Recognised antigenPhospho-SMAD4 (pT277)
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Host animalRabbit (Oryctolagus cuniculus)
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ClonalityPolyclonal (rabbit origin)
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Species reactivityHuman (Homo sapiens) ; Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur.
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Tested applicationsIHC, IF, DB, ELISA
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Recommended dilutionsImmunofluorescence: 1:10-1:50,Dot blot: 1:500,IHC (Paraffin): 1:10-1:50
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NotesTitration of the phospho-SMAD4 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
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Intented useThis phospho-SMAD4 antibodyis to be used only for research purposes and not for diagnostics..
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UniprotQ13485
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PurityAntigen affinity
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DescriptionCommon mediator of signal transduction by TGF-beta (transforming growth factor) superfamily, SMAD4 is the common SMAD (co-SMAD). It promotes binding of the SMAD2/SMAD4/FAST-1 complex to DNA and provides an activation function required for SMAD1 or SMAD2 to stimulate transcription. It may act as a tumor suppressor.
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ImmunogenThis phospho-SMAD4 antibody was produced from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding pT277 of human SMAD4.
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StorageAliquot the phospho-SMAD4 antibody and store frozen at -20 deg. Celcius or lower. Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity. Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized.
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PropertiesIf you buy Antibodies supplied by NJS poly they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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French translationanticorps
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Gene target
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Gene symbolSMAD4
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Short nameAnti-Phospho-SMAD4 (pT277)
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TechniqueAntibody, antibodies against human proteins, antibodies for
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IsotypeRabbit Ig
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Alternative nameAntibodies to Phospho-SMAD4 (pT277)
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Alternative techniqueantibodies
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Alternative to gene targetSMAD family member 4, DPC4 and JIP and MADH4 and MYHRS, SMAD4 and IDBG-3485 and ENSG00000141646 and 4089, transforming growth factor beta receptor, nuclei, Smad4 and IDBG-157503 and ENSMUSG00000024515 and 17128, SMAD4 and IDBG-644730 and ENSBTAG00000006919 and 540248
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Gene info
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Identity
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Gene
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Long gene nameSMAD family member 4
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Synonyms gene
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Synonyms gene name
- MAD, mothers against decapentaplegic homolog 4 (Drosophila)
- SMAD, mothers against DPP homolog 4 (Drosophila)
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Synonyms
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GenBank acession
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Locus
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Discovery year1996-11-15
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- SMAD family
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VEGA ID
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Locus Specific Databases
MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data