USP25 Antibody

• Catalog number
  6143-100
• Price
  Please ask
• Size
  100 μg

• Antibody Target
  USP25
• Target Alternative Name
  USP25, USP21
• Antibody Type
  Polyclonal
• Species Reactivities
  Human, mouse, rat
• Immunogen Sequence
  Fusion protein of human USP25
• Accession
  Q9UHP3
• Gene Id
  29761
• Appearance
  Colorless liquid
• Form
  Liquid
• Formulation
  100 µg of antibody in 100 µl PBS containing 0.02% sodium azide, 50% glycerol, pH 7.3
• Purification
  Affinity purified
• Application
  Western blot
• Positive Control
  Western Blot: BxPC-3 cell lysate
• Application And Usages
  Western blot: 1:500 – 1:1000 dilution. However, the optimal conditions should be determined individually.
• Description
  Ubiquitinating enzymes (UBEs) catalyze protein ubiquitination, a reversible process countered by deubiquitinating enzymes (DUB) action. There are five DUB subfamilies including the USP, UCH, OTU, MJD, and JAMM enzymes. USP25 (ubiquitin specific peptidase 25), also known as USP21, is a 1087 amino acid member of the peptidase C19 family. Expressed at high amounts in pancreas, kidney, skeletal muscle, testis, liver, heart, brain and lung, USP25 associates with the 26S proteasome and functions as a deubiquitinating enzyme in the Ub proteolytic pathway. Due to alternative splicing events, USP25 is expressed as two short, ubiquitously expressed isoforms and one long, muscle specific isoform. The long isoform of USP25 is upregulated in myogenesis and is implicated in the pathogenesis of severe myopathies.
• Storage Condition
  -20 °C
• Shipping Condition
  gel pack
• Shelf Life
  12 months
• Usage
  For Research Use Only! Not to be used in humans.
• Handling
  The antibody solution should be gently mixed before use.
• Western Blot Verified
  Verified
• Immunocytochemistry Verified
  Not Verified
• Immunofluorescence Verified
  Not Verified
• Immunoprecipitation Verified
  Not Verified
• ELISA Verified
  Not Verified
• ChIP Verified
  Not Verified
• Flow Cytometry Verified
  Not Verified
• Data Sheet Link
• Properties
  If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
• French translation
  anticorps

• Gene target
  USP25  
• Gene symbol
  USP25
• Short name
  USP25 Antibody
• Technique
  Antibody, antibodies against human proteins, antibodies for
• Isotype
  Rabbit IgG
• Alternative name
  USP25 (antibody to-)
• Alternative technique
  antibodies

Gene info

• Identity
  HGNC:12624
• Gene
  USP25
• Long gene name
  ubiquitin specific peptidase 25
• Synonyms gene name
  • ubiquitin specific protease 25
• Synonyms
  • USP21
• GenBank acession
  • AF170562
• Locus
  21q21.1
• Discovery year
  2000-02-10
• Entrez gene record
  29761
• Pubmed identfication
  • 12838346
  • 10612803
• Classification
  • Ubiquitin specific peptidases
• VEGA ID
  OTTHUMG00000074343

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

Product images

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