Human CASP3(Caspase 3) ELISA Kit

• Catalog number
  ELK1527
• Price
  Please ask
• Size
  96T

• Alternative Names
  CPP32; CPP32B; SCA1; Apoptain; Yama; Apoptosis-Related Cysteine Peptidase; Cysteinyl Aspartate Specific Proteinases 3; SREBP cleavage activity 1
• Assay Type
  Sandwich
• Sensitivity
  0.056ng/mL
• Standard
  10ng/mL
• Detection range
  0.156-10ng/mL
• Assay length
  3h
• Research Area
  Signal transduction;Enzyme & Kinase;Apoptosis;Tumor immunity;Infection immunity;
• Test principle
  The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Caspase 3 (CASP3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Caspase 3 (CASP3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Caspase 3 (CASP3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Caspase 3 (CASP3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• Description
  Human and some mouse caspases are active in apoptosis and cell death and even in necrosis and inflammation. CASP Gene and orthologous enzymes have been identifies successfully in the signal transduction cascade and pathways.
• Properties
  E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays, E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays, Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
• Test
  ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED

• Gene target
  CASP3   Caspase  
• Gene symbol
  CASP3
• Short name
  CASP3(Caspase 3) ELISA Kit
• Technique
  ELISA kit, ELISA, ELISAs Enzyme-linked immunosorbent assay 90320007 SNOMED code are used by the medical researcher for detection of human, mouse, … proteins are supplied in coated 96 well plates to be stored at +4°C. ELISA test kits can be sandwich ELISA. In sandwich ELISAS Enzyme-linked immunosorbent assay 9we use captor and detector antibodies. In single antibody ELISAs the antigen is coated and only a detector antibody is used. Traditional competition antigen ELISAs are coated with a captor antibody and a competitive antigen is labelled with the chromogen. In this case the ratio on your graph will be inverse proportional with the antigen present in your sample. A sign detected by the Colorimetric detection OD optical density absorbance at 450 nm between 0 and 4 with an ELISA reader for 450 nm absortion. Genprice Inc. the recognized authority tax ID 45-4304622 D-U-N-S number - 078440800. MeSH code E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay. PROMT code 3841327 Immunoabsorbent assay tests. ELISA tests are enzyme linked immunoassays to detect human, mouse or other proteins in serum, plasma, urine or biological fluids. MeSH code E05 478 566 350 170 for Enzyme-Linked Immunosorbent Assay or ELISA kits. Also Enzyme-linked immunoassay, competitive have the SNOMED name 61461007 and the PROMT code for ELISA readers is 3841330. This is the desigation of other diagnostic analyzers and tests.
• Host
  antigen assay ELISA kit 1 Kit (96 Wells)
• Species
  Human, Humans
• Alternative name
  H. sapiens caspase 3, apoptosis-related cysteine peptidase(caspase 3) Enzyme-linked immunosorbent assay reagent
• Alternative technique
  elisas, kits
• Alternative to gene target
  v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832

Gene info

• Identity
  HGNC:1504
• Gene
  CASP3
• Long gene name
  caspase 3
• Synonyms gene name
  • caspase 3, apoptosis-related cysteine protease
  • caspase 3, apoptosis-related cysteine peptidase
• Synonyms
  • CPP32
  • CPP32B
  • Yama
  • apopain
• GenBank acession
  • BC016926
• Locus
  4q35.1
• Discovery year
  1996-07-22
• Entrez gene record
  836
• Pubmed identfication
  • 8780721
• RefSeq identity
  • NM_004346
• Classification
  • Caspases
  • Small nucleolar RNA protein coding host genes
• VEGA ID
  OTTHUMG00000133681

MeSH Data

• Name
  Enzyme-Linked Immunosorbent Assay
• Concept
  Scope note: An immunoassay utilizing an antibody labeled with an enzyme marker such as horseradish peroxidase. While either the enzyme or the antibody is bound to an immunosorbent substrate, they both retain their biologic activity; the change in enzyme activity as a result of the enzyme-antibody-antigen reaction is proportional to the concentration of the antigen and can be measured spectrophotometrically or with the naked eye. Many variations of the method have been developed.
• Tree numbers
  • E05.478.566.350.170
  • E05.478.566.380.360
  • E05.478.583.400.170
  • E05.601.470.350.170
  • E05.601.470.380.360
• Qualifiers
  ethics, mortality, psychology, trends, veterinary, history, classification, economics, instrumentation, methods, nursing, standards, adverse effects, statistics & numerical data

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