GalNAc-T3, soluble fragment, Human Recombinant

• Catalog number
  P1216-5
• Price
  Please ask
• Size
  5 ug

• Synonyms
  Polypeptide N-acetylgalactosaminyltransferase 3, Polypeptide GalNAc transferase 3, GaNTase 3
• Alternative_names
  Polypeptide N-acetylgalactosaminyltransferase 3, Polypeptide GalNAc transferase 3, GaNTase 3
• Description
  Catalyzes the initial reaction in O-linked oligosaccharide biosynthesis
• Recombinant
  Yes
• Source
  Insect cells
• Purity by SDS PAGE
  > 95%
• Assay
  SDS-PAGE
• Activity Specifications test method
  Measured by transfer of N-acetylgalatosamine (GalNac) from UDP-Gal to the peptide EA2, as measured with a phosphatase-coupled assay.
• Biological activity
  Specific activity >500 pmol/min/ug
• Results
  >500 pmol/min/ug
• Storage Temp
  -20°C
• Shipping
  Gel Pack
• Shelf Life
  12 months
• Concentration
  466 ug/ml
• Appearance
  Liquid
• Physical form description
  Sterile filtered solution in 25 mM Tris pH7.5 and 150 mM NaCl
• Background Information
  Polypeptide N-acetylgalactosaminyltransferase 3 (GalNAc-T3) catalyzes the transfer of N-acetylgalactosamine (GalNAc) from UDP-GalNAc to the hydroxyl group of serine and threonine residues. Twenty GalNAc-T genes have been identified in humans and most have been shown to represent active GalNAc-Ts. All isoforms are type II transmembrane proteins, with different but partly overlapping substrate preferences. The GalNAc-Ts control the initiation of mucin-type O-linked glycosylation and determine the location and density of O-glycans in a protein. Addition of GalNAc to an unglycosylated Ser/Thr residue creates the Tn antigen GalNAcα1-S/T, and subsequent addition of sialic acid by ST6GalNAc-I forms the cancer associated STn antigen. GalNAc-T3 is highly expressed in pancreas and testis, and weakly expressed in a few other organs such as placenta. The expression levels of GalNAc-T3 were found to be upregulated in highly differentiated types of adenocarcinoma cell lines as opposed to less differentiated types. Overexpression of GalNAc-T3 has also been found to promote pancreatic cancer cell growth. Mutations in GalNAc-T3 were found to be involved in familial tumoral calcinosis, which may be mediated through glycosylation of fibroblast growth factor 23, protecting it from proteolytic cleavage. GalNAc-T3 has a preference for mono- or diglycosylated substrates.
• Handling
  Centrifuge the vial prior to opening.
• Usage
  For Research Use Only! Not to be used in humans
• Datasheet
• Properties
  Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
• Additional source
  Recombinants or rec. proteins
• Group
  recombinants

• Gene target
  GalNAc-T3   soluble  
• Gene symbol
  HBA1, B4GALT3, B3GNT3, GALNT3, B4GALNT3, SLC25A5, B3GALNT1, TRAF3IP1
• Short name
  GalNAc-T3, soluble , Recombinant
• Technique
  Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
• Isotype
  Antibody fragments and scFv fragments, single chain variable fragments can be E. coli expressed. Fragment peptides for polyclonals give monoclonal like antibodies as the epitope is very small. IgG Fc and(ab)2 fragments are small and stable subunits of the antibody obtained by enzymatic digestion. Complement fragments, bp DNA fragments are common.
• Species
  Human, Humans
• Alternative name
  GalNAc-T3, soluble fragment, H. sapiens Rec.
• Alternative technique
  rec

Gene info

• Identity
  HGNC:4823
• Gene
  HBA1
• Long gene name
  hemoglobin subunit alpha 1
• Synonyms gene name
  • hemoglobin, alpha 1
• Synonyms
  • HBA-T3
• GenBank acession
  • AF349571
• Locus
  16p13.3
• Discovery year
  2001-06-22
• Entrez gene record
  3039
• Pubmed identfication
  • 1975428
  • 2649166
• RefSeq identity
  • NM_000558
• Classification
  • Hemoglobin subunits
• VEGA ID
  OTTHUMG00000060138
• Locus Specific Databases
  • HbVar: A Database of Human Hemoglobin Variants and Thalassemias
  • The Globin Gene Server
  • LRG_1225

Gene info

• Identity
  HGNC:926
• Gene
  B4GALT3
• Long gene name
  beta-1,4-galactosyltransferase 3
• Synonyms gene name
  • UDP-Gal:betaGlcNAc beta 1,4- galactosyltransferase, polypeptide 3
• Synonyms
  • beta4Gal-T3
• GenBank acession
  • BC006099
• Locus
  1q23.3
• Discovery year
  1998-12-02
• Entrez gene record
  8703
• Pubmed identfication
  • 9405390
  • 9597550
• RefSeq identity
  • NM_003779
• Classification
  • Beta 4-glycosyltransferases
• VEGA ID
  OTTHUMG00000034348

Gene info

• Identity
  HGNC:13528
• Gene
  B3GNT3
• Long gene name
  UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 3
• Synonyms gene
  • TMEM3
• Synonyms
  • B3GN-T3
  • beta3Gn-T3
  • HP10328
  • B3GNT-3
• Synonyms name
  • putative type II membrane protein
  • beta-1,3-N-acetylglucosaminyltransferase bGnT-3
  • transmembrane protein 3
• GenBank acession
  • AB015630
• Locus
  19p13.11
• Discovery year
  2000-09-20
• Entrez gene record
  10331
• Pubmed identfication
  • 10072769
  • 11042166
• RefSeq identity
  • NM_014256
• Classification
  • Beta 3-glycosyltransferases
• VEGA ID
  OTTHUMG00000183490

Gene info

• Identity
  HGNC:4125
• Gene
  GALNT3
• Long gene name
  polypeptide N-acetylgalactosaminyltransferase 3
• Synonyms gene name
  • UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase 3 (GalNAc-T3)
• Synonyms
  • GalNAc-T3
  • HHS
  • HFTC
• Synonyms name
  • polypeptide GalNAc transferase 3
• Locus
  2q24.3
• Discovery year
  1996-10-26
• Entrez gene record
  2591
• Pubmed identfication
  • 9592121
  • 15133511
• RefSeq identity
  • NM_004482
• Classification
  • Polypeptide N-acetylgalactosaminyltransferases
• VEGA ID
  OTTHUMG00000132157

Gene info

• Identity
  HGNC:24137
• Gene
  B4GALNT3
• Long gene name
  beta-1,4-N-acetyl-galactosaminyltransferase 3
• Synonyms
  • B4GalNac-T3
  • FLJ16224
  • FLJ40362
• GenBank acession
  • AB089940
• Locus
  12p13.33
• Discovery year
  2006-01-08
• Entrez gene record
  283358
• Pubmed identfication
  • 12966086
• RefSeq identity
  • NM_173593
• Classification
  • Beta 4-glycosyltransferases
• VEGA ID
  OTTHUMG00000129283

Gene info

• Identity
  HGNC:10991
• Gene
  SLC25A5
• Long gene name
  solute carrier family 25 member 5
• Synonyms gene
  • ANT2
• Synonyms gene name
  • solute carrier family 25 (mitochondrial carrier; adenine nucleotide translocator), member 5
• Synonyms
  • T2
  • 2F1
  • T3
• GenBank acession
  • BC068199
• Locus
  Xq24
• Discovery year
  1990-08-03
• Entrez gene record
  292
• Pubmed identfication
  • 2168878
  • 2829183
• RefSeq identity
  • NM_001152
• Classification
  • Solute carriers
• VEGA ID
  OTTHUMG00000022715
• Locus Specific Databases
  • Global Variome shared LOVD

Gene info

• Identity
  HGNC:918
• Gene
  B3GALNT1
• Long gene name
  beta-1,3-N-acetylgalactosaminyltransferase 1 (globoside blood group)
• Synonyms gene
  • B3GALT3
• Synonyms gene name
  • UDP-Gal:betaGlcNAc beta 1,3-galactosyltransferase, polypeptide 3 (Globoside blood group)
  • UDP-GalNAc:betaGlcNAc beta 1,3-galactosaminyltransferase, polypeptide 1 (Globoside blood group)
• Synonyms
  • beta3Gal-T3
  • galT3
  • P1
  • GLOB
• Synonyms name
  • globoside synthase
  • P antigen synthase
• GenBank acession
  • Y15062
• Locus
  3q26.1
• Discovery year
  1998-12-01
• Entrez gene record
  8706
• Pubmed identfication
  • 9582303
  • 10993897
• RefSeq identity
  • NM_033167
• Classification
  • Beta 3-glycosyltransferases
  • Blood group antigens
• VEGA ID
  OTTHUMG00000159064
• Locus Specific Databases
  • LRG_820

Gene info

• Identity
  HGNC:17861
• Gene
  TRAF3IP1
• Long gene name
  TRAF3 interacting protein 1
• Synonyms gene name
  • TNF receptor-associated factor 3 interacting protein 1
• Synonyms
  • MIP-T3
  • DKFZP434F124
  • MIPT3
  • IFT54
  • FAP116
• Synonyms name
  • microtubule interacting protein that associates with TRAF3
• GenBank acession
  • AF230877
• Locus
  2q37.3
• Discovery year
  2004-03-12
• Entrez gene record
  26146
• Pubmed identfication
  • 10791955
  • 12935900
  • 28154159
• RefSeq identity
  • NM_015650
• Classification
  • IFT-B2 complex
• VEGA ID
  OTTHUMG00000152872

MeSH Data

• Name
  Primary Cell Culture
• Concept
  Scope note: The initial culturing of cells derived directly from fresh TISSUES.
• Tree numbers
  • E01.370.225.500.223.500
  • E05.200.500.265.500
  • E05.242.223.500
  • E05.481.500.249.500
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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