Target of this antibody:
Alternative names of antibody target:
Keratin type II cytoskeletal 8, Cytokeratin-8, CK-8, Keratin-8, K8, KRT8, CYK8, KO, CK8, K2C8, CARD2
Keratin 8 (KRT8) belongs to the type B (basic) subfamily of high molecular weight keratins and exists in combination with keratin 18. Keratin 8 is primarily found in the non-squamous epithelia and is present in majority of adenocarcinomas and ductal carcinomas. It is absent in squamous cell carcinomas. Hepatocellular carcinomas are defined by the use of antibodies that recognize only cytokeratin polypeptides 8 and 18.
Clonality of antibody:
This antibody reacts with:
A peptide corresponding to the C-terminus of Human Cytokeratin 5
Form of antibody:
Formulation of this antibody:
In 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Affinity purified using protein A
This antibody has been succesfully tested for use in following methods:
IHC: 1:100 dilution; WB: 1:1000 dilution
Shelf time: 12 months in temp.: -20 deg.C
How to handle this antibody:
The antibody solution should be gently mixed before use.
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.Rabbits are used for polyclonal antibody production by Biovision. Rabbit antibodies are very stable and can be stored for several days at room temperature. Biovision adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
anti-Anti-Cytokeratin 5, Rabbit Monoclonal Antibody
Antibody, Rabbit, anti
antibody to-Anti-Cytokeratin 5, production species: rabbit monoclonal (Antibody to)