Anti-Mouse I-Ap (public; Ia.m57), FITC (Clone 7-16.17) (mouse IgG2a)
-
Catalog number
GEN520365
-
Price
Please ask
-
Size
100ug
-
-
Also known as
I-Ap (public; Ia.m57)
-
Other names
N/A
-
Category
Antibodies
-
Subcategory
Mnoclonal antibodies
-
Gene name
N/A
-
Gene name synonims
N/A
-
Other gene names
N/A
-
Clonality
Monoclonal
-
Immunoglobulin isotype
Mouse IgG2a
-
Clone
7-16.17
-
Host organism
Mouse (Mus musculus)
-
Species reactivity
Mouse (Mus musculus); Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby.
-
Specificity and cross reactivity
I-Ap (Mouse-I-Ap,k,q,r,s,b); Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited. This particular antibody might cross react with speacies outside of the listed ones.
-
Purification method
N/A
-
Form Appearance
FITC (FITC conjugated Ig buffered in PBS, 0.02% NaN3 and EIA grade BSA as a stabilizing protein to bring total protein concentration to 4-5 mg/ml. (Purified from ascitic fluid via Protein G Chromatography))
-
Concentration
Antibody Concentration: 0.1 miligram per 1 mililiter
-
Storage and shipping
Store the antibody at +4 degrees Celsius. For long term storage, aliquot and freeze unused portion the antibody should be stored at -20 degrees Celsius. in volumes appropriate for single usage. Avoid freeze/thaw cycles. Avoid prolonged exposure to light.
-
Tested applications
Immunohistochemistry (IHC)
-
Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
-
Properties
This MBS Monoclonals Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. MBS Monoclonals FITC is highly sensitive to pH extremes.
-
Conjugation
Anti-FITC Antibody
-
Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
-
Latin name
Mus musculus
-
Gene target
-
Gene symbol
ESB3, PIRC88, PIRC83, IFNR, PIRC84, PIRC86
-
Short name
Anti-Mouse I-Ap (public; Ia m57), FITC (Clone 7-16 17) (mouse IgG2a)
-
Technique
anti-, Mouse, anti, FITC, antibody to, antibodies, Fluorescein, mouses
-
Host
mouse
-
Isotype
IgG2a
-
Label
AP
-
Species
Mouse, Mouses
-
Alternative name
Anti-Anti-Mouse I-Ap (public; Ia.m57), FITC (Clone 7-16.17) (mouse IgG2a)
-
Alternative technique
murine, antibodies, fluorescine
-
Clone name
7-16
-
Gene info
-
Identity
-
Gene
-
Long gene name
esterase B3
-
Locus
-
Discovery year
2001-06-22
-
Pubmed identfication
Gene info
Gene info
Gene info
Gene info
Gene info
MeSH Data
-
Name
-
Concept
Scope note:
Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
-
Tree numbers
- E01.370.225.500.607.512.240
- E01.370.225.750.551.512.240
- E05.200.500.607.512.240
- E05.200.750.551.512.240
- E05.478.583.375
-
Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Product images
Similar products
