p300 Inhibitor Screening Assay Kit (Fluorometric)

• Catalog number
  K346-100
• Price
  Please ask
• Size
  100 assays

• Description
  Simple method to screen p300 Inhibitors
• Summary
  • Detection method- Fluorescence (Ex/Em = 392/482 nm) • Applications- Screening/studying/characterizing potential inhibitors of p300
• Detection Method
  Fluorescence (Ex/Em = 392/482 nm)
• Applications
  Screening/studying/characterizing potential inhibitors of p300
• Features Benefits
  • Simple procedure; takes ~ 45 min • Fast and convenient • Kit contains the necessary reagents for testing putative p300 inhibitors
• Storage Conditions
  -80°C
• Shipping Conditions
  dry ice
• Shelf life
  12 months
• Background
  p300 (EC 2.3.1.32) or KAT3B, a histone acetyltransferase, contributes to transcriptional activation by acetylating chromatin on the lysine residues of H3 and H4 histones. There is growing evidence that p300 plays an important role in cancer cell proliferation and differentiation. p300 inhibitors have potential applications in cancer therapy. BioVision’s p300 Inhibitor Screening Kit utilizes a H3 peptide and Acetyl CoA as the substrates. p300 acetylates the peptide and generates CoA-SH with a free thiol group. The CoA is detected using a Thiol Detecting Probe that reacts with thiol groups and gives enhanced fluorescence that can be measured at Ex/Em = 392/482 nm. In the presence of p300 specific inhibitors, the enzymatic activity is inhibited resulting in decreased or total loss of fluorescence. This assay kit is a simple, sensitive, and rapid tool to screen potential inhibitors of p300.
• Data Sheet Link
• Test
  Biovision supplies other types of Assays as 1.
• Additional description
  Tissue, pathway, proteinase, peptidase, protease ,acrosin, lipoprotein, activator, caspase, trypsin, papain, esterase inhibitors are proteins or receptor ligands or receptor antagonists that bind to an enzyme receptor and decreases its activity. Since blocking an enzyme's activity can kill a pathogen or correct a metabolic imbalance, many drugs are enzyme inhibitors. Not all receptor antagonist that bind to enzymes are inhibitors; enzyme activator ligands or agonists bind to enzymes and increase their enzymatic activity, while enzyme substrates bind and are converted to products in the normal catalytic cycle of the enzyme.

• Gene target
  p300   Inhibitor   Screening   Kit   Fluorometric  
• Short name
  p300 Inhibitor Screening Assay Kit (Fluorometric)
• Technique
  Assay, assays
• Host
  Assay
• Alternative name
  p300 suppressor detection test reagent (fluorescence)
• Alternative technique
  arrays, kits
• Alternative to gene target
  v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832

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