Hoechst 33342

• Catalog number
  H456605
• Price
  Please ask
• Size
  100 mg

• Chemical available in other sizes
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• Stock availability
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• Cas number
  23491-52-3
• Chemical s molecular weight
  452.55
• Chemical s main applications
  Hoechst 33342 is an A:T-specific DNA minor groove ligand that is a widely used fluorochrome for visualizing cellular DNA. Excitation of the dye occurs around 350 nm with an emission maxima around 461 nm. Hoechst-33342, unlike other Hoechst dyes, is more lipophillic due to an additional ethyl group. The compound has been observed to displace several known DNA intercalators. Adriamycin has been shown to disrupt the DNA dye update. The compound can be used in sorting living cells based on DNA content by FACS, in the visualization of chromatin distribution in living cells, to detect BrdU incorporation into cells, the visualization of fixed cells by microscopy, and to study the initial stages of apoptosis and cell-cycle distribution. In addition to Hoechst-33342's fluorescent capability, the chemical has also had varying effects on differing cell types. The stain has been shown to induce cell differentiation, bind reversibly to Friend erythroleukemia cells, inhibit Topo I (topoisomerase I), induce apoptosis in HL-60 cells, and in junctional sarcoplasmic reticulum cells regulate Ca2+ efflux and affect cell differentiation.
• Other name
  2'-(4-Ethoxyphenyl)-5-(4-methyl-1-piperazinyl)-2,5'-bi-1H-benzimidazole; 2-[2-(4-Ethoxyphenyl)-6-benzimidazolyl]-6-(1-methyl-4-piperazinyl)benzimidazole; BXI 72; Bisbenzimide; HOE 33342; Ho 342; Hoechst H 33342; NSC 334072; 2'-(p-Ethoxyphenyl)-5-(4-methyl-1-piperazinyl)-2,5'-bibenzimidazole
• Chemical s formula
  C27H28N6O
• Physical properties
  No Data Available
• Melting temperature
  No Data Available
• Boiling temperature
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• Chemical s soluble in
  No Data Available
• Stability conditions
  No Data Available
• Storage
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• Chemical s specifications

• Gene target
  Hoechst   33342  
• Short name
  Hoechst 33342
• Alternative name
  Hoechst 33342

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