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Available ordering format
Inquire
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Immunogen
BSA-conjugated molecule: 5-hydroxymethylcytosine (5-hmC)
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Raised in
Mouse
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Clonality
Monoclonal
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Clone
IgG1k
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Purification
Affinity purified
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How to reconstitute
See included datasheet or contact our support service
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Storage condition
Store at -20°C; for long term storage -80°C is recommened; make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tubes.
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Verified applications
Dot blot (Dot), ELISA (ELISA), hMeDIP (hydroxymethylated DNA immunoprecipitation)
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Connected products
collection of antibodies to epigenetics
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Recommended dilutions for use
2 µg/ml (Dot), 1: 500 (ELISA), 2.5 µg/IP (hMeDIP)
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Molecular weight expected аpparent
see datasheet
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Verified reactivity
human
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Possible reactivity
mouse, broad species range
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No reactivity
No confirmed exceptions from predicted reactivity known at the moment
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Supplementary information
To be added when available
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References
To be added when available, antibody released in February 2016.
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Scientific context
5-hmC | 5-hydroxymethylcytosine is a recently discovered DNA modification which results from the enzymatic conversion of 5-methylcytosine into 5-hydroxymethylcytosine by the TET family of oxygenases. It may have an important roles distinct from that of 5-methylcytosine (5-mC). 5-hmC bases have been identified in Purkinje neurons, in granule cells and embryonic stem cells where they are present at high levels (up to 0,6% of total nucleotides in Purkinje cells.
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Notes
Close structural similarity between 5-mC and 5-hmC makes them very difficult to expreimentally distinuish. Therefore affinity-based technology was employed to purify 5-hmC specific antibodies. This antibody is affinity purified and provided in PBS pH 7.4 with 0.05 % sodium azide.
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Protein number
NA
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TAIR number
Refer to NCBI
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About
Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
