PUMA Antibody

• Catalog number
  R30371
• Price
  Please ask
• Size
  0.1mg

• Category
  Antibody
• Concentration
  0.5mg/ml if reconstituted with 0.2ml sterile DI water
• Form
  Antigen affinity purified
• Conjugation
  Unconjugated
• Clone
  Polyclonal antibody
• Recognised antigen
  PUMA
• Host animal
  Rabbit (Oryctolagus cuniculus)
• Clonality
  Polyclonal (rabbit origin)
• Species reactivity
  Human (Homo sapiens), Mouse (Mus musculus), Rat ; Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur.
• Tested applications
  WB
• Recommended dilutions
  Western blot: 0.5-1ug/ml
• Notes
  The stated application concentrations are suggested starting amounts. Titration of the PUMA antibody may be required due to differences in protocols and secondary/substrate sensitivity.
• Intented use
  This PUMA antibodyis to be used only for research purposes and not for diagnostics..
• Uniprot
  Q9BXH1
• Purity
  Antigen affinity
• Description
  The p53 upregulated modulator of apoptosis, or PUMA, is a pro-apoptotic member of the Bcl-2 protein family. The PUMA gene is located at 19q. PUMA transcript is contained within 4 exons, with the presumptive initiation codon in exon 2. The predicted 193-amino acid PUMA protein shares 91% amino acid identity with the murine sequence. Bcl-2 family members can form hetero- or homodimers, and they act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. The expression of PUMA is regulated by the tumor suppressor p53, and PUMA has been shown to be involved in p53-mediated apoptosis. Additionally, PUMA encodes 2 BH3 domain-containing proteins, PUMA-alpha and PUMA-beta, that are produced through the use of an alternative first exon and are induced in cells following p53 activation. Furthermore, PUMA couples the nuclear and cytoplasmic proapoptotic functions of p53.
• Immunogen
  An amino acid sequence from the C-terminus of human PUMA (ADDLNAQYERRRQEE) was used as the immunogen for this PUMA antibody (100% homologous in human, mouse and rat).
• Storage
  After reconstitution, the PUMA antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20 deg. Celcius or lower. Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity. Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized. Avoid repeated freezing and thawing.
• Properties
  If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
• French translation
  anticorps

• Gene target
  PUMA  
• Gene symbol
  BBC3, HCAR2
• Short name
  Anti-PUMA
• Technique
  Antibody, antibodies against human proteins, antibodies for
• Isotype
  Rabbit IgG
• Alternative name
  Antibodies to PUMA
• Alternative technique
  antibodies

Gene info

• Identity
  HGNC:17868
• Gene
  BBC3
• Long gene name
  BCL2 binding component 3
• Synonyms
  • JFY1
  • PUMA
• Synonyms name
  • p53-upregulated modulator of apoptosis
• GenBank acession
  • AF332558
• Locus
  19q13.32
• Discovery year
  2002-07-22
• Entrez gene record
  27113
• Pubmed identfication
  • 11463392
  • 11572983
• RefSeq identity
  • NM_014417
• Classification
  • BCL2 homology region 3 (BH3) only
  • MicroRNA protein coding host genes
• VEGA ID
  OTTHUMG00000183489

Gene info

• Identity
  HGNC:24827
• Gene
  HCAR2
• Long gene name
  hydroxycarboxylic acid receptor 2
• Synonyms gene
  • GPR109A
• Synonyms gene name
  • G protein-coupled receptor 109A
• Synonyms
  • HCA2
  • HM74A
  • PUMAG
  • Puma-g
  • NIACR1
• Synonyms name
  • niacin receptor 1
• GenBank acession
  • AY148884
• Locus
  12q24.31
• Discovery year
  2004-04-22
• Entrez gene record
  338442
• Pubmed identfication
  • 21167710
  • 12522134
  • 12646212
  • 19141678
  • 18983141
  • 21454438
• RefSeq identity
  • NM_177551
• Classification
  • Hydroxycarboxylic acid receptors
• VEGA ID
  OTTHUMG00000162722

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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