LEO1 Antibody, ALEXA FLUOR 350

• Catalog number
  bs-10030R-A350
• Price
  Please ask
• Size
  100ul

• Long name
  LEO1 Polyclonal Antibody, ALEXA FLUOR 350 Conjugated
• Also known as
  Anti-LEO1 PAb ALEXA FLUOR 350
• Category
  Conjugated Primary Antibodies
• Conjugated with
  ALEXA FLUOR® 350
• Host Organism
  Rabbit (Oryctolagus cuniculus)
• Target Antigen
  LEO1
• Specificity
  This is a highly specific antibody against LEO1.
• Modification
  Unmodified
• Modification Site
  None
• Clonality
  Polyclonal
• Clone
  Polyclonal antibody
• Concentration
  1ug per 1ul
• Immunogen range
  500-550/666
• Subcellular location
  Nucleus
• Source
  This antibody was obtained by immunization of the host with KLH conjugated synthetic peptide derived from human LEO1
• Gene ID Number
  123169
• Swiss Prot
  Q8WVC0
• Tested applications
  IF(IHC-P)
• Recommended dilutions
  IF(IHC-P)(1:50-200)
• Crossreactivity
  Human, Mouse, Rat
• Cross reactive species details
  Due to limited amount of testing and knowledge, not every possible cross-reactivity is known.
• Background of the antigen
  Component of the PAF1 complex (PAF1C) which has multiple functions during transcription by RNA polymerase II and is implicated in regulation of development and maintenance of embryonic stem cell pluripotency. PAF1C associates with RNA polymerase II through interaction with POLR2A CTD non-phosphorylated and 'Ser-2'- and 'Ser-5'-phosphorylated forms and is involved in transcriptional elongation, acting both indepentently and synergistically with TCEA1 and in cooperation with the DSIF complex and HTATSF1. PAF1C is required for transcription of Hox and Wnt target genes. PAF1C is involved in hematopoiesis and stimulates transcriptional activity of KMT2A/MLL1; it promotes leukemogenesis through association with KMT2A/MLL1-rearranged oncoproteins, such as KMT2A/MLL1-MLLT3/AF9 and KMT2A/MLL1-MLLT1/ENL. PAF1C is involved in histone modifications such as ubiquitination of histone H2B and methylation on histone H3 'Lys-4' (H3K4me3). PAF1C recruits the RNF20/40 E3 ubiquitin-protein ligase complex and the E2 enzyme UBE2A or UBE2B to chromatin which mediate monoubiquitination of 'Lys-120' of histone H2B (H2BK120ub1); UB2A/B-mediated H2B ubiquitination is proposed to be coupled to transcription. PAF1C is involved in mRNA 3' end formation probably through association with cleavage and poly(A) factors. In case of infection by influenza A strain H3N2, PAF1C associates with viral NS1 protein, thereby regulating gene transcription. Involved in polyadenylation of mRNA precursors. Connects PAF1C to Wnt signaling.
• Purification
  Purified by Protein A.
• Storage conditions
  Store this antibody in aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Keep refrigerated at 2 to 8 degrees Celcius for up to one year.
• Excitation emission
  343nm/442nm
• Synonyms
  RDL; RNA polymerase-associated protein LEO1; Replicative senescence down-regulated leo1-like protein; LEO1
• Properties
  For facs or microscopy Alexa 1 conjugate. Alexa Fluor 350 conjugates can be used in multi-color flow cytometry with FACS's equipped with a second red laser or red diode. If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies. ALEXA FLUOR they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
• Conjugation
  Alexa Fluor, ALEXA FLUOR 350
• French translation
  anticorps

• Gene target
  LEO1  
• Gene symbol
  LEO1
• Short name
  Anti-LEO1
• Technique
  Antibody, antibodies against human proteins, antibodies for
• Isotype
  Immunoglobulin G (IgG)
• Label
  ALEXA FLUOR 350
• Alternative name
  LEO1 Polyclonal Antibody, ALEXA FLUOR 350
• Alternative technique
  antibodies

Gene info

• Identity
  HGNC:30401
• Gene
  LEO1
• Long gene name
  LEO1 homolog, Paf1/RNA polymerase II complex component
• Synonyms gene name
  • Leo1, Paf1/RNA polymerase II complex component, homolog (S. cerevisiae)
• GenBank acession
  • AY302186
• Locus
  15q21.2
• Discovery year
  2005-07-20
• Entrez gene record
  123169
• Pubmed identfication
  • 15632063
  • 24038468
• RefSeq identity
  • NM_138792
• Classification
  • Paf1/RNA polymerase II complex
• VEGA ID
  OTTHUMG00000131843

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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