Recombinant Mouse CHK2 / CHEK2 Protein (His & GST tag)

• Catalog number
  PKSM040303-20µg
• Price
  Please ask
• Size
  20µg

• Synonym
  Cds1;CHK2;HUCDS1;Rad53
• Activity
  Kinase activity untested
• Sequence
  Mey 1-Leu 546
• Fusion tag
  N-His & GST
• Accession
  Q9Z265
• Expressed Host
  Baculovirus-Insect Cells
• Shipping
  Kinases are highly recommended to be shipped at frozen temperature with blue ice or dry ice.Shipment made at ambient temperature may seriously affect the activity of the ordered products.
• Purity
  > 90 % as determined by SDS-PAGE
• Endotoxin
  < 1.0 EU per μg of the protein as determined by the LAL method
• Stability and Storage
  Samples are stable for up to twelve months from date of receipt at -70℃.Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Mol Mass
  89 kDa
• AP Mol Mass
  90 kDa
• Formulation
  Supplied as sterile 20mM Tris, 500mM NaCl, pH 8.0
• Background
  In response to DNA damage and replication blocks, cell cycle progression is halted through the control of critical cell cycle regulators. The protein encoded by CHEK2 gene is a cell cycle checkpoint regulator and putative tumor suppressor. It contains a forkhead-associated protein interaction domain essential for activation in response to DNA damage and is rapidly phosphorylated in response to replication blocks and DNA damage. When activated, the encoded CHEK2 protein is known to inhibit CDC25C phosphatase, preventing entry into mitosis, and has been shown to stabilize the tumor suppressor protein p53, leading to cell cycle arrest in G1. In addition, this protein interacts with and phosphorylates BRCA1, allowing BRCA1 to restore survival after DNA damage. Mutations in this gene have been linked with Li-Fraumeni syndrome, a highly penetrant familial cancer phenotype usually associated with inherited mutations in TP53. Also, mutations in CHEK2s gene are thought to confer a predisposition to sarcomas, breast cancer, and brain tumors. This nuclear protein is a member of the CDS1 subfamily of serine/threonine protein kinases. Several transcript variants encoding different isoforms have been found for this gene. Immune Checkpoint   Immunotherapy   Cancer Immunotherapy   Targeted Therapy
• Technical data
• Conjugation
  histidine
• Test
  Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
• Latin name
  Mus musculus
• Source
  Recombinants or rec. proteins
• Group
  recombinants

• Gene target
  CHK2   CHEK2   Protein   His   GST   tag  
• Gene symbol
  CHEK2, MGST3
• Short name
  Recombinant Mouse CHK2 / CHEK2 Protein (His & GST tag)
• Technique
  Recombinant, Mouse, mouses, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Elabscience advises they will be reconstituted in a buffer soluion or culture medium for cell culture. Tags
• Host
  mouse
• Label
  His
• Species
  Mouse, Mouses
• Alternative name
  Rec. Mouse CHK2 / checkpoint kinase 2 Protein (histidine & GST detection labelled)
• Alternative technique
  rec, murine
• Alternative to gene target
  checkpoint kinase 2, CDS1 and CHK2 and hCds1 and HuCds1 and LFS2 and PP1425 and RAD53, CHEK2 and IDBG-3314 and ENSG00000183765 and 11200, transferase activity, nuclei, Chek2 and IDBG-190635 and ENSMUSG00000029521 and 50883, CHEK2 and IDBG-636030 and ENSBTAG00000004956 and 518897

Gene info

• Identity
  HGNC:16627
• Gene
  CHEK2
• Long gene name
  checkpoint kinase 2
• Synonyms gene
  • RAD53
• Synonyms gene name
  • CHK2 (checkpoint, S.pombe) homolog
  • CHK2 checkpoint homolog (S. pombe)
• Synonyms
  • CDS1
  • CHK2
  • HuCds1
  • PP1425
  • bA444G7
• GenBank acession
  • AF086904
• Locus
  22q12.1
• Discovery year
  2001-09-19
• Entrez gene record
  11200
• Pubmed identfication
  • 9836640
  • 10097108
• RefSeq identity
  • NM_001005735
• VEGA ID
  OTTHUMG00000151023
• Locus Specific Databases
  • LRG_302

Gene info

• Identity
  HGNC:7064
• Gene
  MGST3
• Long gene name
  microsomal glutathione S-transferase 3
• Synonyms
  • GST-III
• Synonyms name
  • microsomal glutathione S-transferase III
  • microsomal GST-3
  • microsomal GST-III
• GenBank acession
  • AF026977
• Locus
  1q24.1
• Discovery year
  1997-10-16
• Entrez gene record
  4259
• Pubmed identfication
  • 9278457
• RefSeq identity
  • NM_004528
• Classification
  • Microsomal glutathione S-transferases
• VEGA ID
  OTTHUMG00000034627

MeSH Data

• Name
  Primary Cell Culture
• Concept
  Scope note: The initial culturing of cells derived directly from fresh TISSUES.
• Tree numbers
  • E01.370.225.500.223.500
  • E05.200.500.265.500
  • E05.242.223.500
  • E05.481.500.249.500
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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