Taglines A protein that controls proliferation, cellular differentiation, & other functions in most cells
Gene Source Human
Source Human 293 cell expressed
Endotoxin Level<1.0 EU per 1 μg of protein
Activity (Specifications/test method) N/A
Biological activity ED₅₀ is typically 0.1 to 0.5 ng/mL. The specific activity was determined by the dose-dependent inhibition of IL-4 induced proliferation of mouse HT-2 cells (BALB/c spleen activated by sheep erythrocytes in the presence of IL-2).
Results 0.1 to 0.5 ng/ml
Molecular Weight 25 kDa, homodimer, nonglycosylated
Storage Temp. -20°C
Shipping gel pack
Shelf Life 1 year
Physical form description Lyophilized from 50 mM sodium acetate, pH 4.5 and 350 mM NaCl.
Reconstitution Instructions Reconstitute in sterile 4 mM HCl containing 0.1% endotoxin-free recombinant human serum albumin.
Handling Centrifuge the vial prior to opening.
Usage For Research Use Only! Not to be used in humans
Properties Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Additional source Recombinants or rec. proteins